ArticleF1000Research2018
A method for transplantation of human HSCs into zebrafish, to replace humanised murine transplantation models.
Article in F1000Research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 25 citations in OpenAlex.
- PDX models for functional precision oncology and discovery science.Nature reviews. Cancer · 2025Review
- Macrophage depletion overcomes human hematopoietic cell engraftment failure in zebrafish embryo.Cell death & disease · 2024Article
- Homing and Engraftment of Hematopoietic Stem Cells Following Transplantation: A Pre-Clinical Perspective.Current oncology (Toronto, Ont.) · 2024Review
- Hematopoietic Stem and Progenitor Cell Identification and Transplantation in Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Chemically-defined generation of human hemogenic endothelium and definitive hematopoietic progenitor cells.Biomaterials · 2022Article
- Article
- Review
- Targeted therapy of human leukemia xenografts in immunodeficient zebrafish.Scientific reports · 2021Article
- Humanized zebrafish enhance human hematopoietic stem cell survival and promote acute myeloid leukemia clonal diversity.Haematologica · 2020Article
- Reporting animal research: Explanation and elaboration for the ARRIVE guidelines 2.0.PLoS biology · 2020Article
- Review
- CRISPR/Cas9-mediatedHaematologica · 2020Article
- Partial loss of actin nucleator actin-related protein 2/3 activity triggers blebbing in primary T lymphocytes.Immunology and cell biology · 2020Article
- Determining macrophage versus neutrophil contributions to innate immunity using larval zebrafish.Disease models & mechanisms · 2020Review
- Calcio-Herbal Medicine Divya-Swasari-Vati Ameliorates SARS-CoV-2 Spike Protein-Induced Pathological Features and Inflammation in Humanized Zebrafish Model by Moderating IL-6 and TNF-α Cytokines.Journal of inflammation research · 2020Article
- Zebrafish for Personalized Regenerative Medicine; A More Predictive Humanized Model of Endocrine Disease.Frontiers in endocrinology · 2020Review
- CRISPR/Cas9-modified hematopoietic stem cells-present and future perspectives for stem cell transplantation.Bone marrow transplantation · 2019Review
- A method for transplantation of human HSCs into zebrafish, to replace humanised murine transplantation models.F1000Research · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Haematopoietic stem cell (HSC) transplantation is a critical therapy for haematopoietic malignancies and immune disorders. Incomplete or delayed engraftment of HSCs in the host results in increased risk of infection and morbidity. The mechanisms of HSC engraftment are poorly understood and understanding these processes will increase transplantation success on many levels. Current animal models are immunocompromised 'humanised' mice transplanted with human HSCs. Harmful procedures include genetic manipulations and irradiation to ablate the mouse immune system, and opaque mouse tissues make visualisation of the early steps of HSC engraftment impossible. There is a need for new models to offer alternatives to humanised mice in the study of HSC transplantation. Here we described a detailed method for transplantation of human HSCs into zebrafish, before the onset of adaptive immunity. Human HSCs were purified from whole blood by enrichment of the CD34 cell population using a positive magnetic selection and further purified using an anti-CD34 antibody and cell sorting. Sorted CD34 cells were transplanted into the blood stream of 52 hour old zebrafish larvae. Human HSCs home into the zebrafish haematopoietic niche, where they engage with endothelial cells and undergo cell division. Our model offers the opportunities to image
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.