ArticleScientific reports2018
The atypical subunit composition of respiratory complexes I and IV is associated with original extra structural domains in Euglena gracilis.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 15 citations in OpenAlex.
- Euglena gracilis Subcellular Fractionation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- The mitochondrial proteome of diplonemids: from conventional pathways to eccentric RNA editing and transcript processing.BMC genomics · 2025Article
- Mitochondrion-to-nucleus communication mediated by RNA export: a survey of potential mechanisms and players across eukaryotes.Biology letters · 2024Review
- Antares I: a Modular Photobioreactor Suitable for Photosynthesis and Bioenergetics Research.Applied biochemistry and biotechnology · 2024Article
- Euglena's atypical respiratory chain adapts to the discoidal cristae and flexible metabolism.Nature communications · 2024Article
- Functional and biochemical characterization of the Toxoplasma gondii succinate dehydrogenase complex.PLoS pathogens · 2023Article
- The biogenesis and regulation of the plant oxidative phosphorylation system.Plant physiology · 2023Review
- Trophic state alters the mechanism whereby energetic coupling between photosynthesis and respiration occurs in Euglena gracilis.The New phytologist · 2021Article
- Supramolecular associations between atypical oxidative phosphorylation complexes of Euglena gracilis.Journal of bioenergetics and biomembranes · 2021Article
- Article
- Specific features and assembly of the plant mitochondrial complex I revealed by cryo-EM.Nature communications · 2020Article
- A Uniquely Complex Mitochondrial Proteome from Euglena gracilis.Molecular biology and evolution · 2020Article
- Evolution of metabolic capabilities and molecular features of diplonemids, kinetoplastids, and euglenids.BMC biology · 2020Article
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
In mitochondrial oxidative phosphorylation, electron transfer from NADH or succinate to oxygen by a series of large protein complexes in the inner mitochondrial membrane (complexes I-IV) is coupled to the generation of an electrochemical proton gradient, the energy of which is utilized by complex V to generate ATP. In Euglena gracilis, a non-parasitic secondary green alga related to trypanosomes, these respiratory complexes totalize more than 40 Euglenozoa-specific subunits along with about 50 classical subunits described in other eukaryotes. In the present study the Euglena proton-pumping complexes I, III, and IV were purified from isolated mitochondria by a two-steps liquid chromatography approach. Their atypical subunit composition was further resolved and confirmed using a three-steps PAGE analysis coupled to mass spectrometry identification of peptides. The purified complexes were also observed by electron microscopy followed by single-particle analysis. Even if the overall structures of the three oxidases are similar to the structure of canonical enzymes (e.g. from mammals), additional atypical domains were observed in complexes I and IV: an extra domain located at the tip of the peripheral arm of complex I and a "helmet-like" domain on the top of the cytochrome c binding region in complex IV.
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