Evidence map›Paper›PMID 29944202›Full record

ArticleCurrent protocols in cytometry2018

Multiphoton Intravital Calcium Imaging.

Claire E J Cheetham

Abstract read
In one paragraph

Article in Current protocols in cytometry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Claire E J CheethamDepartment of Neurobiology, University of Pittsburgh, Pittsburgh, Pennsylvania.ORCID 0000-0002-4038-1501

Funding

Functional Integration of Newborn Olfactory Sensory Neurons in the Healthy and Regenerating Olfactory SystemR01DC018516 · NIDCD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CHEETHAM, CLAIRE ELIZABETH JANE · 2021 to 2025
$1.8M
The role of immature olfactory sensory neurons in olfactory processing and plasticityR03DC014788 · NIDCD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CHEETHAM, CLAIRE ELIZABETH JANE · 2015 to 2017
$459k
NIDCD NIH HHS R01 DC018516NIDCD NIH HHS R03 DC014788
6 · The paper itself

Abstract

Multiphoton intravital calcium imaging is a powerful technique that enables high-resolution longitudinal monitoring of cellular and subcellular activity hundreds of microns deep in the living organism. This unit addresses the application of 2-photon microscopy to imaging of genetically encoded calcium indicators (GECIs) in the mouse brain. The protocols in this unit enable real-time intravital imaging of intracellular calcium concentration simultaneously in hundreds of neurons, or at the resolution of single synapses, as mice respond to sensory stimuli or perform behavioral tasks. Protocols are presented for implantation of a cranial imaging window to provide optical access to the brain and for 2-photon image acquisition. Protocols for implantation of both open skull and thinned skull windows for single or multi-session imaging are described. © 2018 by John Wiley & Sons, Inc.

Indexed as

BrainAnimalsCalcium SignalingIntravital MicroscopyMiceMice, TransgenicMicroscopy, Fluorescence, MultiphotonSynapses2-photon microscopybraincranial windowgenetically-encoded calcium indicatorintravital imagingin vivo imagingmultiphoton imaging

Identifiers

PMID29944202
PMCPMC6039231

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.