Evidence map›Paper›PMID 29943354›Full record

ArticleIn vitro cellular & developmental biology. Animal2018

VPA selectively regulates pluripotency gene expression on donor cell and improve SCNT embryo development.

Xinxin Li, Xudong Ao, Li Bai, Dongfang Li, Xuefei Liu, Zhuying Wei, Shorgan Bou, Guangpeng Li

Abstract read
PubMed Publisher
In one paragraph

Article in In vitro cellular & developmental biology. Animal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Epigenetic Reprogramming and Somatic Cell Nuclear Transfer.Methods in molecular biology (Clifton, N.J.) · 2023
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xinxin LiState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China.
Xudong AoState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China.
Li BaiState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China.
Dongfang LiState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China.
Xuefei LiuState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China.
Zhuying WeiState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China.
Shorgan BouState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China.
Guangpeng LiState Key Laboratory of Reproductive Regulation & Breeding of Grassland Livestock, Inner Mongolia University, 24 Zhaojun Road, Hohhot, 010021, Inner Mongolia Autonomous Region, People's Republic of China. gpengli@imu.edu.cn.

Funding

National Basic Research Program of China 973 Program-2012CB22306National Key Project for Production of Transgenic Livestock No.2011ZX08007-002
6 · The paper itself

Abstract

SCNT technology has been successfully used to clone a variety of mammals, but the cloning efficiency is very low. This low efficiency is likely due to the incomplete reprogramming of SCNT embryos. Histone modification and DNA methylation may participate in these events. Thus, it would be interesting to attempt to improve the efficiency of SCNT by using a HDACi VPA. In order to guarantee the effect of VPA and reduce its cytotoxicity, a comprehensive analysis of the cell proliferation and histone modification was performed. The results showed that 0.5 and 1 mM VPA treatment for 24 h were the optimal condition. According to the results, H3K4me3 was increased in 0.5 and 1 mM VPA groups, whereas H3K9me2 was significantly decreased. These are the signals of gene-activation. In addition, VPA treatment led to the overexpression of Oct4 and Nanog. These indicated that VPA-treated cells had similar patterns of histone to zygotic embryos, and may be more favorable for reprograming. A total of 833 cloned embryos were produced from the experimental replicates of VPA-treated donor cells. In 1 mM treatment group, the blastocyst rates were significantly increased compared with control. At the same time, our findings demonstrated the interrelation between DNA methylation and histone modifications.

Indexed as

Nuclear Transfer TechniquesAnimalsCattleCloning, OrganismDNA MethylationEmbryonic DevelopmentFetusFibroblastsGene Expression Regulation, DevelopmentalHistonesPluripotent Stem CellsPromoter Regions, GeneticProtein Processing, Post-TranslationalValproic AcidHistonesValproic AcidDNA methylationEmbryo developmentHistone modificationSCNTVPA

Identifiers

PMID29943354

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.