ArticlePlant methods2018
Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips.
Article in Plant methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Cryopreservation of Rubus viruses in raspberry shoot tips via droplet-vitrification: assessment of viral preservation, localization, and post-thaw transmission capacity.Plant methods · 2025Article
- Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Eradication ofFrontiers in plant science · 2022Article
- Preserving plant samples from remote locations for detection of RNA and DNA viruses.Frontiers in microbiology · 2022Article
- Epigenetic and Genetic Integrity, Metabolic Stability, and Field Performance of Cryopreserved Plants.Plants (Basel, Switzerland) · 2021Review
- Osmo- and cryotherapy of sugarcane (Virusdisease · 2020Article
- Single-wall carbon nanotubes improve cell survival rate and reduce oxidative injury in cryopreservation ofPlant methods · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPreservation of plant virus is a fundamental requirement in all types of virus-related research and applied applications. Development of efficient, reliable strategies for long-term preservation of plant virus would largely assist these studies.
resultsThe present study reported a novel biotechnology allowing cryopreservation of Apple stem grooving virus (ASGV) in living shoot tips. Following cryopreservation by droplet-vitrification or encapsulation-dehydration, about 62-67% of shoot regrowth and 100% of ASGV cryopreservation were obtained. Although shoot proliferation and virus concentration were reduced in cryopreserved diseased shoots after 8 weeks of shoot regeneration, continuous subculture for 4 times (16 weeks) increased shoot proliferation and virus concentration to comparative levels as those produced by shoot tip culture (as a control to shoot tip cryopreservation). Cryopreserved ASGV was efficiently transmitted to a woody plant by micrografting and to a herbaceous indicator by mechanical inoculation. Gene sequencing in three fragments of ASGV genome including coat protein and movement protein showed that cryopreserved ASGV shared 99.87% nucleotide identities with shoot tip culture-preserved virus, indicating cryopreserved virus is genetically stable.
conclusionsThe present study demonstrates ASGV, a representative virus that can infect meristematic cells of shoot tips, can be efficiently cryopreserved in shoot tips. To the best of our knowledge, this is the first report on plant virus cryopreservation in living tissues, and has great potential applications to long-term preservation of plant viruses.
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