Evidence map›Paper›PMID 29941940›Full record

ArticleCell death & disease2018

NLRP3 inflammasome is expressed and regulated in human islets.

Fanny Lebreton, Ekaterine Berishvili, Géraldine Parnaud, Caroline Rouget, Domenico Bosco, Thierry Berney, Vanessa Lavallard

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell death & disease, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Fanny LebretonCell Isolation and Transplantation Center, Department of Surgery, Geneva University, Hospitals and University of Geneva, Geneva, Switzerland.
Ekaterine BerishviliCell Isolation and Transplantation Center, Department of Surgery, Geneva University, Hospitals and University of Geneva, Geneva, Switzerland.
Géraldine ParnaudCell Isolation and Transplantation Center, Department of Surgery, Geneva University, Hospitals and University of Geneva, Geneva, Switzerland.
Caroline RougetCell Isolation and Transplantation Center, Department of Surgery, Geneva University, Hospitals and University of Geneva, Geneva, Switzerland.
Domenico BoscoCell Isolation and Transplantation Center, Department of Surgery, Geneva University, Hospitals and University of Geneva, Geneva, Switzerland.
Thierry BerneyCell Isolation and Transplantation Center, Department of Surgery, Geneva University, Hospitals and University of Geneva, Geneva, Switzerland.
Vanessa LavallardCell Isolation and Transplantation Center, Department of Surgery, Geneva University, Hospitals and University of Geneva, Geneva, Switzerland. vanessa.lavallard@unige.ch.ORCID 0000-0001-7328-7549
University of Geneva · CH

Funding

Juvenile Diabetes Research Foundation Australia (JDRF Australia) 3-RSC-2016-162-I-XSchweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) 310030-173138Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung (Swiss National Science Foundation) 31003A_170090/1
6 · The paper itself

Abstract

NRLP3 inflammasome is a protein complex involved in the maturation of IL1β. In the onset of type 1 diabetes as well as in islet transplantation, IL-1β is one of the cytokines involved in the recruitment of immune cells in islets and eventually in islet destruction. Whether IL-1β is produced by islet cells is still under debate and NLRP3 inflammasome-dependent IL-1β production has not been yet determined in human islets. The aim of this study was to determine the expression and the regulation of the NRLP3 inflammasome in human islets. Human islets were stimulated with LPS and successively with ATP (LPS + ATP) in the presence or absence of the inflammasome inhibitor glyburide. Islets were also incubated in hypoxic or normoxic conditions for 24 h in the presence or absence of glyburide. Then, IL1B and NLRP3 expression was studied by real time PCR, protein expression by western blot, protein localization by immunofluorescence and protein secretion by ELISA. LPS + ATP increased gene expression of NRLP3 and IL1B. Glyburide partially prevented this effect. IL-1β protein was localized in β and non-β cells. Moreover, LPS + ATP increased IL-1β protein expression and production, which were prevented by glyburide. Hypoxia increased gene expression of NRLP3 and IL1B and induced IL-1β and caspase-1 production. Finally, hypoxia-induced cell death which was not prevented by inhibition of NLRP3 inflammasome. NRLP3 inflammasome is expressed and plays a role in IL-1β production by human islets. By contrast, NRLP3 inflammasome activation is not involved in islet cell death induced by hypoxia.

Indexed as

Gene Expression RegulationCell DeathGlyburideHumansHypoxiaInflammasomesInterleukin-1betaIslets of LangerhansNLR Family, Pyrin Domain-Containing 3 ProteinGlyburideInflammasomesInterleukin-1betaNLR Family, Pyrin Domain-Containing 3 Protein

Identifiers

PMID29941940
PMCPMC6018156
OpenAlexW2809524316

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.