ArticleScientific reports2018
Ablation of Runx2 in Ameloblasts Suppresses Enamel Maturation in Tooth Development.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Integrative multi-omics and bioinformatic analysis ofThe Japanese dental science review · 2026Review
- Ufl1-Mediated UFMylation Sustains Amelogenesis by Stabilizing RUNX2.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026Article
- A splicing-derived microRNA from amelogenin exon4 regulates enamel formation via control of exon4 splicing and amelogenin expression.Scientific reports · 2026Article
- The Interplay of Immune Response and Dental Enamel Formation in Health and Sickness.Advances in experimental medicine and biology · 2026Review
- Review
- Study protocol on the impact of postnatal maternal separation stress on dental enamel formation in a murine experimental model.PloS one · 2024Article
- Enam expression is regulated by Msx2.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Dental characteristics of patients with four different types of skeletal dysplasias.Clinical oral investigations · 2023Article
- Article
- Mediator 1 ablation induces enamel-to-hair lineage conversion in mice through enhancer dynamics.Communications biology · 2023Article
- Odontogenesis-Associated Phosphoprotein (ODAPH) Overexpression in Ameloblasts Disrupts Enamel Formation via Inducing Abnormal Mineralization of Enamel in Secretory Stage.Calcified tissue international · 2022Article
- Enamel biomineralization under the effects of indomethacin and celecoxib non-steroidal anti-inflammatory drugs.Scientific reports · 2022Article
- Loss of BMP2 and BMP4 Signaling in the Dental Epithelium Causes Defective Enamel Maturation and Aberrant Development of Ameloblasts.International journal of molecular sciences · 2022Article
- An miRNA derived from amelogenin exon4 regulates expression of transcription factor Runx2 by directly targeting upstream activators Nfia and Prkch.The Journal of biological chemistry · 2022Article
- The synergistic effects of TGF-β1 and RUNX2 on enamel mineralization through regulating ODAPH expression during the maturation stage.Journal of molecular histology · 2022Article
- The Association Between Runx Signaling and Craniofacial Development and Disease.Current osteoporosis reports · 2022Review
- Advanced materials for enamel remineralization.Frontiers in bioengineering and biotechnology · 2022Review
- Deficiency of Mineralization-Regulating Transcription Factor Trps1 Compromises Quality of Dental Tissues and Increases Susceptibility to Dental Caries.Frontiers in dental medicine · 2022Article
- Integration of multimodal data in the developing tooth reveals candidate regulatory loci driving human odontogenic phenotypes.Frontiers in dental medicine · 2022Article
- Runx2 deficiency in junctional epithelium of mouse molars decreases the expressions of E-cadherin and junctional adhesion molecule 1.Journal of molecular histology · 2021Article
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Authors and funding
11 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Runt-related transcription factor 2 (Runx2) is involved in the early stage of tooth development. However, only few studies have reported the role of Runx2 in enamel development, which may be attributed to that Runx2 full knockout mice cannot survive after birth. In the present study, we successfully established a Runx2-deficient mouse model using a conditional knockout (cKO) method. We observed a significant reduction in the degree of mineralization and the decreased size of enamel rods in cKO mice. Histological analysis showed the retained enamel proteins in enamel layer at maturation stage in cKO molars. Further analysis by qRT-PCR revealed that the expressions of genes encoding enamel structure proteins, such as amelogenin (AMELX), ameloblastin (AMBN) and enamelin (ENAM), were increased in cKO enamel organs. On the other hand, the expression of kallikrein-related peptidase-4 (KLK4) at the mRNA and protein levels was dramatically decreased from late secretory stage to maturation stage in cKO enamel organs, while the expression of matrix metalloproteinase-20 (MMP-20) was not significantly altered. Finally, immunohistochemistry indicated that the uptake of amelogenins by ameloblasts was significantly decreased in cKO mice. Taken together, Runx2 played critical roles in controlling enamel maturation by increasing synthesis of KLK4 and decreasing synthesis of AMELX, AMBN and ENAM.
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