Evidence map›Paper›PMID 29941214›Full record

ArticleUltrasound in medicine & biology2018

Inactivation of Planktonic Escherichia coli by Focused 1-MHz Ultrasound Pulses with Shocks: Efficacy and Kinetics Upon Volume Scale-Up.

Andrew A Brayman, Brian E MacConaghy, Yak-Nam Wang, Keith T Chan, Wayne L Monsky, Valery P Chernikov, Sergey V Buravkov, Vera A Khokhlova, Thomas J Matula

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Ultrasound in medicine & biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Histotripsy-Induced Bactericidal Activity Correlates to Size of Cavitation Cloud In Vitro.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2024
    Article
  2. Histotripsy: A Method for Mechanical Tissue Ablation with Ultrasound.Annual review of biomedical engineering · 2024
    Review
  3. Article
  4. Review
  5. Article
  6. The histotripsy spectrum: differences and similarities in techniques and instrumentation.International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2023
    Review
  7. Article
  8. Treating Porcine Abscesses with Histotripsy: A Pilot Study.Ultrasound in medicine & biology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Andrew A BraymanCenter for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA. Electronic address: mailbrayman@apl.washington.edu.
Brian E MacConaghyCenter for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA.
Yak-Nam WangCenter for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA.
Keith T ChanTacoma Radiology Associates, Tacoma WA, USA.
Wayne L MonskyDepartment of Radiology, University of Washington, Seattle, WA, USA.
Valery P ChernikovResearch Institute of Human Morphology, Laboratory of Cell Pathology, Moscow, Russia.
Sergey V BuravkovFaculty of Fundamental Medicine, M.V. Lomonosov Moscow State University, Moscow, Russia.
Vera A KhokhlovaCenter for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA; Department of Acoustics, Physics Faculty, M.V. Lomonosov Moscow State University, Moscow, Russia.
Thomas J MatulaCenter for Industrial and Medical Ultrasound, Applied Physics Laboratory, University of Washington, Seattle, WA, USA.

Funding

Nonlinear Acoustics in Calibration/Metrology of High Intensity Focused UltrasoundR01EB007643 · NIBIB · UNIVERSITY OF WASHINGTON · PI KHOKHLOVA, VERA · 2008 to 2020
$6.9M
HIFU for Noninvasive Abscess TreatmentR01EB019365 · NIBIB · UNIVERSITY OF WASHINGTON · PI MATULA, THOMAS · 2015 to 2018
$2.6M
NIBIB NIH HHS R01 EB007643NIBIB NIH HHS R01 EB019365
6 · The paper itself

Abstract

This study addresses inactivation of E. coli in either 5- or 10-mL volumes, which were 50- to 100-fold greater than used in an earlier study (Brayman et al. 2017). Cells were treated with 1-MHz pulsed high-intensity focused ultrasound (10 cycles, 2-kHz repetition frequency, +65/-12.8 MPa focal pressures). The surviving fraction was assessed by coliform assay, and inactivation demonstrated curvilinear kinetics. The reduction of surviving fraction to 50% required 2.5 or 6 min in 5- or 10-mL samples, respectively. Exposure of 5 mL for 20 min reduced the surviving fraction to ∼1%; a similar exposure of 10-mL samples reduced the surviving fraction to ∼10%. Surviving cells from 5-min exposures appeared normal under light microscopy, with minimal debris; after 20 min, debris dominated. Transmission electron microscopy images of insonated samples showed some undamaged cells, a few damaged but largely intact cells and comminuted debris. Cellular damage associated with substantive but incomplete levels of inactivation can be variable, ranging from membrane holes tens of nanometers in diameter to nearly complete comminution.

Indexed as

Escherichia coliHigh-Energy Shock WavesPlanktonCells, CulturedCell SurvivalKineticsMicroscopy, Electron, TransmissionAcoustic bacterial inactivationEscherichia coli inactivationFocused ultrasoundLight microscopy of bacterial HIFU lysateNon-linear waveform distortionPlanktonic bacterial inactivationPulsed HIFUShock frontsTEM of bacterial HIFU lysateUltrasonic bacterial inactivation

Identifiers

PMID29941214
PMCPMC6135241

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.