ArticleBMC plant biology2018
Mapping QTLs for water-use efficiency reveals the potential candidate genes involved in regulating the trait in apple under drought stress.
Article in BMC plant biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Drought stress priming induces morphological adaptation and methylome changes in apple seedlings.BMC plant biology · 2026Article
- Integrated BSA-seq and RNA-seq analyses revealFrontiers in plant science · 2026Article
- Development of KASP markers assisted with soybean drought tolerance in the germination stage based on GWAS.Frontiers in plant science · 2024Article
- 4-methylumbelliferone (4-MU) enhances drought tolerance of apple by regulating rhizosphere microbial diversity and root architecture.Horticulture research · 2023Article
- Construction of the first high-density genetic linkage map and QTL mapping of flavonoid and leaf-size related traits in Epimedium.BMC plant biology · 2023Article
- Natural alleles of the abscisic acid catabolism gene ZmAbh4 modulate water use efficiency and carbon isotope discrimination in maize.The Plant cell · 2022Article
- Single marker analysis for leaf gas exchange traits from RILS of RD 23 (O. sativa L.) and O. longistaminata.Biotechnology reports (Amsterdam, Netherlands) · 2022Article
- Molecular sexual determinants in Pistacia genus by KASP assay.Molecular biology reports · 2022Article
- Fine-tuning of SUMOylation modulates drought tolerance of apple.Plant biotechnology journal · 2022Article
- Engineering drought-tolerant apple by knocking down sixHorticulture research · 2022Article
- Identification of PLATZ genes inFrontiers in plant science · 2022Article
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- Apple SERRATE negatively mediates drought resistance by regulating MdMYB88 and MdMYB124 and microRNA biogenesis.Horticulture research · 2020Article
- Apple whole genome sequences: recent advances and new prospects.Horticulture research · 2019Review
- Small and genetically highly structured populations in a long-legged bee, Rediviva longimanus, as inferred by pooled RAD-seq.BMC evolutionary biology · 2018Article
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9 authors.
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Abstract
backgroundImprovement of water-use efficiency (WUE) can effectively reduce production losses caused by drought stress. A better understanding of the genetic determination of WUE in crops under drought stress has great potential value for developing cultivars adapted to arid regions. To identify the genetic loci associated with WUE and reveal genes responsible for the trait in apple, we aim to map the quantitative trait loci (QTLs) for carbon isotope composition, the proxy for WUE, applying two contrasting irrigating regimes over the two-year experiment and search for the candidate genes encompassed in the mapped QTLs.
resultsWe constructed a high-density genetic linkage map with 10,172 markers of apple, using single nucleotide polymorphism (SNP) markers obtained through restriction site-associated DNA sequencing (RADseq) and a final segregating population of 350 seedlings from the cross of Honeycrisp and Qinguan. In total, 33 QTLs were identified for carbon isotope composition in apple under both well-watered and drought-stressed conditions. Three QTLs were stable over 2 years under drought stress on linkage groups LG8, LG15 and LG16, as validated by Kompetitive Allele-Specific PCR (KASP) assays. In those validated QTLs, 258 genes were screened according to their Gene Ontology functional annotations. Among them, 28 genes were identified, which exhibited significant responses to drought stress in 'Honeycrisp' and/or 'Qinguan'. These genes are involved in signaling, photosynthesis, response to stresses, carbohydrate metabolism, protein metabolism and modification, hormone metabolism and transport, transport, respiration, transcriptional regulation, and development regulation. They, especially those for photoprotection and relevant signal transduction, are potential candidate genes connected with WUE regulation in drought-stressed apple.
conclusionsWe detected three stable QTLs for carbon isotope composition in apple under drought stress over 2 years, and validated them by KASP assay. Twenty-eight candidate genes encompassed in these QTLs were identified. These stable genetic loci and series of genes provided here serve as a foundation for further studies on marker-assisted selection of high WUE and regulatory mechanism of WUE in apple exposed to drought conditions, respectively.
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