ArticlePloS one2018
Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed.
- Extracellular matrix: new insights into its role in female reproductive aging and potential therapeutic strategies.npj aging · 2026Review
- Biophysical Considerations in Cell Fusion.Advances in experimental medicine and biology · 2026Review
- Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures.Scientific reports · 2024Article
- Unveiling the human fetal-maternal interface during the first trimester: biophysical knowledge and gaps.Frontiers in cell and developmental biology · 2024Review
- The reprotoxic adverse side effects of neurogenic and neuroprotective drugs: current use of human organoid modeling as a potential alternative to preclinical models.Frontiers in pharmacology · 2024Review
- Trophoblast Syncytialization: A Metabolic Crossroads.Results and problems in cell differentiation · 2024Article
- Extracellular Matrix Influences Gene Expression and Differentiation of Mouse Trophoblast Stem Cells.Stem cells and development · 2023Article
- Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells.Bioengineering (Basel, Switzerland) · 2023Article
- Screening and functional analysis of the differential peptides from the placenta of patients with healthy pregnancy and preeclampsia using placental peptidome.Frontiers in genetics · 2022Article
- Matrigel® enhances 3T3-L1 cell differentiation.Adipocyte · 2021Article
- Delta-9-tetrahydrocannabinol inhibits invasion of HTR8/SVneo human extravillous trophoblast cells and negatively impacts mitochondrial function.Scientific reports · 2021Article
- Nasal Septum Deviation as the Consequence of BMP-Controlled Changes to Cartilage Properties.Frontiers in cell and developmental biology · 2021Article
- A Novel 3D Culture Model of Human ASCs Reduces Cell Death in Spheroid Cores and Maintains Inner Cell Proliferation Compared With a Nonadherent 3D Culture.Frontiers in cell and developmental biology · 2021Article
- Human Female Reproductive System Organoids: Applications in Developmental Biology, Disease Modelling, and Drug Discovery.Stem cell reviews and reports · 2020Review
- Delta-9-tetrahydrocannabinol disrupts mitochondrial function and attenuates syncytialization in human placental BeWo cells.Physiological reports · 2020Article
- The role of extracellular matrix in normal and pathological pregnancy: Future applications of microphysiological systems in reproductive medicine.Experimental biology and medicine (Maywood, N.J.) · 2020Review
- Establishment of an in vitro placental barrier model cultured under physiologically relevant oxygen levels.Molecular human reproduction · 2020Article
- Mechanobiological regulation of placental trophoblast fusion and function through extracellular matrix rigidity.Scientific reports · 2020Article
- Review
- Microphysiological systems of the placental barrier.Advanced drug delivery reviews · 2020Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The incorporation of the extracellular matrix (ECM) is essential for generating in vitro models that truly represent the microarchitecture found in human tissues. However, the cell-cell and cell-ECM interactions in vitro remains poorly understood in placental trophoblast biology. We investigated the effects of varying the surface properties (surface thickness and stiffness) of two ECMs, collagen I and Matrigel, on placental trophoblast cell morphology, viability, proliferation, and expression of markers involved in differentiation/syncytial fusion. Most notably, thicker Matrigel surfaces were found to induce the self-assembly of trophoblast cells into 3D spheroids that exhibited thickness-dependent changes in viability, proliferation, syncytial fusion, and gene expression profiles compared to two-dimensional cultures. Changes in F-actin organization, cell spread morphologies, and integrin and matrix metalloproteinase gene expression profiles, further reveal that the response to surface thickness may be mediated in part through cellular stiffness-sensing mechanisms. Our derivation of self-assembling trophoblast spheroid cultures through regulation of ECM surface alone contributes to a deeper understanding of cell-ECM interactions, and may be important for the advancement of in vitro platforms for research or diagnostics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.