ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2018
Hard X-Ray Nanoholotomography: Large-Scale, Label-Free, 3D Neuroimaging beyond Optical Limit.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 70 citations in OpenAlex.
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- The Use of Synchrotron Radiation in the Medical Sciences.Photon science · 2026Article
- Synchrotron Radiation-Based Tomography of an Entire Mouse Brain with Sub-Micron Voxels: Augmenting Interactive Brain Atlases with Terabyte Data.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Terbium-doped gadolinium garnet thin films grown by liquid phase epitaxy for scintillation detectors.RSC advances · 2025Article
- Scaling up X-ray holographic nanotomography for neuronal tissue imaging.Biomedical optics express · 2025Article
- High-resolution X-ray phase-contrast tomography of human placenta with different wavefront markers.Scientific reports · 2025Article
- 3D multiscale characterization of the human placenta: Bridging anatomy and histology by X-ray phase-contrast tomography.PNAS nexus · 2025Article
- Crossing length scales: X-ray approaches to studying the structure of biological materials.IUCrJ · 2024Review
- Multi-resolution X-ray phase-contrast and dark-field tomography of human cerebellum with near-field speckles.Biomedical optics express · 2024Article
- Structural and functional imaging of brains.Science China. Chemistry · 2023Review
- X-ray multiscale 3D neuroimaging to quantify cellular aging and neurodegeneration postmortem in a model of Alzheimer's disease.European journal of nuclear medicine and molecular imaging · 2022Article
- Comparative hard x-ray tomography for virtual histology of zebrafish larva, human tooth cementum, and porcine nerve.Journal of medical imaging (Bellingham, Wash.) · 2022Article
- Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke.International journal of biological sciences · 2022Review
- Three-dimensional virtual histology of the cerebral cortex based on phase-contrast X-ray tomography.Biomedical optics express · 2021Article
- Three-dimensional virtual histology of the human hippocampus based on phase-contrast computed tomography.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Article
- Asbestos bodies count and morphometry in bulk lung tissue samples by non-invasive X-ray micro-tomography.Scientific reports · 2021Article
- Multiscale photonic imaging of the native and implanted cochlea.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- 3d phase-contrast nanotomography of unstained human skin biopsies may identify morphological differences in the dermis and epidermis between subjects.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2021Article
- Upscaling X-ray nanoimaging to macroscopic specimens.Journal of applied crystallography · 2021Review
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There have been great efforts on the nanoscale 3D probing of brain tissues to image subcellular morphologies. However, limitations in terms of tissue coverage, anisotropic resolution, stain dependence, and complex sample preparation all hinder achieving a better understanding of the human brain functioning in the subcellular context. Herein, X-ray nanoholotomography is introduced as an emerging synchrotron radiation-based technology for large-scale, label-free, direct imaging with isotropic voxel sizes down to 25 nm, exhibiting a spatial resolution down to 88 nm. The procedure is nondestructive as it does not require physical slicing. Hence, it allows subsequent imaging by complementary techniques, including histology. The feasibility of this 3D imaging approach is demonstrated on human cerebellum and neocortex specimens derived from paraffin-embedded tissue blocks. The obtained results are compared to hematoxylin and eosin stained histological sections and showcase the ability for rapid hierarchical neuroimaging and automatic rebuilding of the neuronal architecture at the level of a single cell nucleolus. The findings indicate that nanoholotomography can complement microscopy not only by large isotropic volumetric data but also by morphological details on the sub-100 nm level, addressing many of the present challenges in brain tissue characterization and probably becoming an important tool in nanoanatomy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.