Evidence map›Paper›PMID 29936684›Full record

ArticleMolecular and cellular biochemistry2019

The effects of L-cysteine and N-acetyl-L-cysteine on homocysteine metabolism and haemostatic markers, and on cardiac and aortic histology in subchronically methionine-treated Wistar male rats.

Sanja Kostić, Žarko Mićovic, Lazar Andrejević, Saša Cvetković, Aleksandra Stamenković, Sanja Stanković, Radmila Obrenović, Milica Labudović-Borović, Dragan Hrnčić, Vladimir Jakovljević and 1 more

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In one paragraph

Article in Molecular and cellular biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed, 1 pooled it
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Sanja KostićFaculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Visegradska 26/II, Belgrade, 11000, Serbia.
Žarko MićovicMilitary Health Department, Ministry of Defence, Belgrade, Serbia.
Lazar AndrejevićClinic of Gynecology and Obstetrics, Faculty of Medical Science University of Pristina - Kosovska Mitrovica, Kosovska Mitrovica, Serbia.
Saša CvetkovićClinic of Gynecology and Obstetrics, Faculty of Medical Science University of Pristina - Kosovska Mitrovica, Kosovska Mitrovica, Serbia.
Aleksandra StamenkovićSt. Boniface Hospital Research Center, Institute of Cardiovascular Sciences, University of Manitoba, Winnipeg, Canada.
Sanja StankovićCentre of Medical Biochemistry, Clinical Centre of Serbia, Belgrade, Serbia.
Radmila ObrenovićCentre of Medical Biochemistry, Clinical Centre of Serbia, Belgrade, Serbia.
Milica Labudović-BorovićFaculty of Medicine, Institute of Histology and Embryology "Aleksandar Dj. Kostic", University of Belgrade, Belgrade, Serbia.
Dragan HrnčićFaculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Visegradska 26/II, Belgrade, 11000, Serbia.
Vladimir JakovljevićDepartment of Physiology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.
Dragan DjurićFaculty of Medicine, Institute of Medical Physiology "Richard Burian", University of Belgrade, Visegradska 26/II, Belgrade, 11000, Serbia. drdjuric@eunet.rs.
University of Belgrade · RSSechenov University · RUSt. Boniface Hospital · CAUniversity of Defence · RS

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Methionine is the precursor of homocysteine, a sulfur amino acid intermediate in the methylation and transsulfuration pathways; methionine-rich diets were used to induce hyperhomocysteinemia, and cardiovascular pathology was often observed. Other sulfur amino acids interfere with this metabolism, i.e., L-cysteine (Cys) and N-aceyl-L-cysteine (NAC), and probably also affect cardiovascular system. Their effects are controversial due to their ability to act both as anti- or pro-oxidant. Thus, this study aimed to elucidate their influence on levels of homocysteine, folate and vitamin B12, levels of different haemostatic parameters (fibrinogen, D-dimer, vWF Ag, vWF Ac) in rat serum or plasma as well as their effects on cardiac and aortic tissue histology in subchronically methionine-treated rats. Wistar albino rats were divided into 4 experimental groups: (a) control group (0.9% sodium chloride 0.1-0.2 mL/day) (n = 10) (K); (b) DL-methionine (0.8 mmol/kg/bw/day) (n = 10) (M); (c) DL-methionine (0.8 mmol/kg/bw/day) + L-cysteine (7 mg/kg/bw/day) (n = 8) (C); (d) DL-methionine (0.8 mmol/ kg/bw/day) + N-acetyl-L-cysteine (50 mg/kg/bw/day) (n = 8) (N). All substances were applied i.p., treatment duration 3 weeks. Lower levels of vitamin B12 in all the groups were found. Folate was reduced only in N group. Decreased fibrinogen was noted in C and N groups and increased D-dimer only in C. VWF activity was reduced in M and C groups. Deleterious effects in heart were observed, especially after Cys and NAC application. Aortic tissue remained unchanged. In conclusion, it could be said that sulfur amino acids have the significant impact on cardiovascular system in subchronically methionine-treated rats. This study points out the relevance of their complex interactions and deleterious effects mediated by either direct influence or procoagulant properties.

Indexed as

AcetylcysteineAnimalsAortaBiomarkersCysteineHeartHemostaticsHomocysteineMaleMethionineRatsRats, WistarAcetylcysteineBiomarkersCysteineHemostaticsHomocysteineMethionineHaemostasisHeartHomocysteineMethionineSulfur amino acids

Identifiers

PMID29936684
OpenAlexW2811135666

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.