Evidence map›Paper›PMID 29936673›Full record

ArticleAMB Express2018

Molecular cloning, expression and adhesion analysis of silent slpB of Lactobacillus acidophilus NCFM.

Yuxing Guo, Xiangyue Li, Yao Yang, Zhen Wu, Xiaoqun Zeng, Fawze Nadari, Daodong Pan

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in AMB Express, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 9 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Yuxing GuoDepartment of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, 210097, People's Republic of China.
Xiangyue LiDepartment of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, 210097, People's Republic of China.
Yao YangDepartment of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, 210097, People's Republic of China.
Zhen WuKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, People's Republic of China.
Xiaoqun ZengKey Laboratory of Animal Protein Deep Processing Technology of Zhejiang Province, Ningbo University, Ningbo, 315211, Zhejiang, People's Republic of China.
Fawze NadariDepartment of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, 210097, People's Republic of China.
Daodong PanDepartment of Food Science & Nutrition, Ginling College, Nanjing Normal University, Nanjing, 210097, People's Republic of China. daodongpan@163.com.
Nanjing Normal University · CNNingbo University · CN

Funding

"333 Project" Foundation of Jiangsu Province BRA2017450Natural Science Funding of China 31471598Natural Science Funding of China 31571852Natural Science Funding of China 31601487Natural Science Funding of China 31671869Natural Science Funding of Jiangsu BK20151544Natural Science Funding of Zhejiang LQ16C200002
6 · The paper itself

Abstract

The slpB gene of Lactobacillus acidophilus NCFM, which differs from the slpA gene and is silent under normal conditions, was successfully amplified and ligated to the corresponding available sites on a recombinant pET-28a vector. Then the pET-28a-slpB vector was transformed into Escherichia coli DH (DE3) and the fusion His-slpB protein was expressed by induction with 1 mM IPTG for 14 h at 37 °C. The resulting His-slpB protein (S

Indexed as

AdhesionCaco-2 cellHis-slpB fusionLactobacillus acidophilus NCFMpET-28a-slpBS-layer protein

Identifiers

PMID29936673
PMCPMC6015585
OpenAlexW2810922516

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.