ArticleMethods (San Diego, Calif.)2018
Morphometric analysis and neuroanatomical mapping of the zebrafish brain.
Article in Methods (San Diego, Calif.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Functional validation of CHMP7 as an ADHD risk gene.Translational psychiatry · 2020Pooled it
- Evolution of a central dopamine circuit underlies adaptation of a light-evoked sensorimotor response in the blind cavefish.Science advances · 2026Article
- A comparative brain atlas in the Mexican cavefish identifies widespread changes in cellular composition and gene expression.bioRxiv : the preprint server for biology · 2026Article
- Pmpcb modulates zebrafish neurogenesis and stress resistance via regulating mitochondria metabolism and function.Molecular neurobiology · 2026Article
- CaMPARI2 enables stimulus-locked whole-brain activity mapping at cellular resolution in unrestrained larval zebrafish.Frontiers in molecular neuroscience · 2026Article
- Visual-acoustic thigmotaxis in zebrafish larvae: a high throughput NAM for neurotoxicity assessment.Frontiers in toxicology · 2026Article
- CaMPARI2 Enables Stimulus-Locked Whole-Brain Activity Mapping at Cellular Resolution in Unrestrained Larval Zebrafish.bioRxiv : the preprint server for biology · 2025Article
- Panx1a modulates metabolic stress signaling and synaptic composition in the developing zebrafish brain.Cell and tissue research · 2025Article
- Development of Zebrafish model for Iron Induced Neuroinflammation.Fish physiology and biochemistry · 2025Article
- Parp1 deletion rescues cerebellar hypotrophy in xrcc1 mutant zebrafish.Scientific reports · 2025Article
- Mutations in the microexon splicing regulator srrm4 have minor phenotypic effects on zebrafish neural development.G3 (Bethesda, Md.) · 2025Article
- Effects of Valproic Acid Embryonic Exposure on Zebrafish: A Systematic Review and Meta-Analysis.NeuroSci · 2024Review
- Evolution of a central dopamine circuit underlies adaptation of light-evoked sensorimotor response in the blind cavefish,bioRxiv : the preprint server for biology · 2024Article
- Short- and Long-Term Neurobehavioral Effects of Developmental Exposure to Valproic Acid in Zebrafish.International journal of molecular sciences · 2024Article
- Brain Imaging and Registration in Larval Zebrafish.Methods in molecular biology (Clifton, N.J.) · 2024Article
- New approach methods to assess developmental and adult neurotoxicity for regulatory use: a PARC work package 5 project.Frontiers in toxicology · 2024Article
- Functional and pharmacological analyses of visual habituation learning in larval zebrafish.eLife · 2023Article
- Article
- TSA-PACT: a method for tissue clearing and immunofluorescence staining on zebrafish brain with improved sensitivity, specificity and stability.Cell & bioscience · 2023Article
- Deficiency of AP1 ComplexInternational journal of molecular sciences · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Large-scale genomic studies have recently identified genetic variants causative for major neurodevelopmental disorders, such as intellectual disability and autism. However, determining how underlying developmental processes are affected by these mutations remains a significant challenge in the field. Zebrafish is an established model system in developmental neurogenetics that may be useful in uncovering the mechanisms of these mutations. Here we describe the use of voxel-intensity, deformation field, and volume-based morphometric techniques for the systematic and unbiased analysis of gene knock-down and environmental exposure-induced phenotypes in zebrafish. We first present a computational method for brain segmentation based on transgene expression patterns to create a comprehensive neuroanatomical map. This map allowed us to disclose statistically significant changes in brain microstructure and composition in neurodevelopmental models. We demonstrate the effectiveness of morphometric techniques in measuring changes in the relative size of neuroanatomical subdivisions in atoh7 morphant larvae and in identifying phenotypes in larvae treated with valproic acid, a chemical demonstrated to increase the risk of autism in humans. These tools enable rigorous evaluation of the effects of gene mutations and environmental exposures on neural development, providing an entry point for cellular and molecular analysis of basic developmental processes as well as neurodevelopmental and neurodegenerative disorders.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.