ArticleVirus research2018
Differential susceptibility of macrophages to serotype II feline coronaviruses correlates with differences in the viral spike protein.
Article in Virus research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- Beyond macrophages: FIPV tropism includes T and B lymphocytes.Veterinary microbiology · 2026Article
- Development and characterization of reverse genetics systems of feline infectious peritonitis virus for antiviral research.Veterinary research · 2024Article
- Myocarditis in an FIP-Diseased Cat with FCoV M1058L Mutation: Clinical and Pathological Changes.Animals : an open access journal from MDPI · 2024Article
- Isolation and Genomic Characteristics of a Novel Pathogenicity Type I Feline Coronavirus in Mainland China.Transboundary and emerging diseases · 2024Article
- An updated review of feline coronavirus: mind the two biotypes.Virus research · 2023Review
- Natural selection differences detected in key protein domains between non-pathogenic and pathogenic Feline Coronavirus phenotypes.bioRxiv : the preprint server for biology · 2023Article
- Natural selection differences detected in key protein domains between non-pathogenic and pathogenic feline coronavirus phenotypes.Virus evolution · 2023Article
- Article
- Detection of Feline Coronavirus Variants in Cats without Feline Infectious Peritonitis.Viruses · 2022Article
- Article
- Feline coronavirus with and without spike gene mutations detected by real-time RT-PCRs in cats with feline infectious peritonitis.Journal of feline medicine and surgery · 2020Article
- FCoV Viral Sequences of Systemically Infected Healthy Cats Lack Gene Mutations Previously Linked to the Development of FIP.Pathogens (Basel, Switzerland) · 2020Article
- Article
- CRFK and Primary Macrophages Transcriptomes in Response to Feline Coronavirus Infection Differ Significantly.Frontiers in genetics · 2020Article
- Middle East Respiratory Syndrome Coronavirus in Dromedaries in Ethiopia Is Antigenically Different From the Middle East Isolate EMC.Frontiers in microbiology · 2019Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to infect and replicate in monocytes/macrophages is a critically distinguishing feature between the two feline coronavirus (FCoV) pathotypes: feline enteric coronavirus (FECV; low-virulent) and feline infectious peritonitis virus (FIPV; lethal). Previously, by comparing serotype II strains FIPV 79-1146 and FECV 79-1683 and recombinant chimeric forms thereof in cultured feline bone marrow macrophages, we mapped this difference to the C-terminal part of the viral spike (S) protein (S2). In view of the later identified diagnostic difference in this very part of the S protein of serotype I FCoV pathotypes, the present study aimed to further define the contribution of the earlier observed ten amino acids difference to the serotype II virus phenotype in macrophages. Using targeted RNA recombination as a reverse genetics system we introduced the mutations singly and in combinations into the S gene and evaluated their effects on the infection characteristics of the mutant viruses in macrophages. While some of the single mutations had a significant effect, none of them fully reverted the infection phenotype. Only by combining five specific mutations the infections mediated by the FIPV and FECV spike proteins could be fully blocked or potentiated, respectively. Hence, the differential macrophage infection phenotype is caused by the cooperative effect of five mutations, which occur in five functionally different domains of the spike fusion subunit S2. The significance of these observations will be discussed, taking into account also some questions related to the identity of the virus strains used.
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