Evidence map›Paper›PMID 29935198›Full record

ArticleJournal of neuroscience methods2018

Refinement of embolic stroke model in rats: Effect of post-embolization anesthesia duration on arterial blood pressure, cerebral edema and mortality.

Bryan T C Chuang, Xiaoguang Liu, Alexander J Lundberg, Tommy J K Toung, John A Ulatowski, Raymond C Koehler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Optimized mouse model of embolic MCAO: From cerebral blood flow to neurological outcomes.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2022
    Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Bryan T C ChuangDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA; Department of Anesthesiology, Saint Mary's Hospital Luodong, Taiwan.
Xiaoguang LiuDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA.
Alexander J LundbergDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA.
Tommy J K ToungDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA.
John A UlatowskiDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA.
Raymond C KoehlerDepartment of Anesthesiology and Critical Care Medicine, Johns Hopkins University, Baltimore, MD, USA. Electronic address: rkoehler@jhmi.edu.
Johns Hopkins Medicine · USJohns Hopkins University · US

Funding

Cell-Free O2 Carriers: Cerebrovascular Control &StrokeR01NS038684 · NINDS · JOHNS HOPKINS UNIVERSITY · PI KOEHLER, RAYMOND CHARLES · 2000 to 2014
$5.6M
Therapeutic Strategies for Neonatal Hypoxic-Ischemiic EncephalopathyR01NS060703 · NINDS · JOHNS HOPKINS UNIVERSITY · PI KOEHLER, RAYMOND CHARLES · 2008 to 2019
$3.9M
Development of real-time, non-invasive photoacoustic imaging to rapidly detect and track perinatal focal stroke at birthR01HL139543 · NHLBI · JOHNS HOPKINS UNIVERSITY · PI BOCTOR, EMAD M, GRAHAM, ERNEST MARSHALL · 2018 to 2021
$2.4M
NHLBI NIH HHS R01 HL139543NINDS NIH HHS R01 NS038684NINDS NIH HHS R01 NS060703
6 · The paper itself

Abstract

backgroundInjection of a clot into the internal carotid artery is an experimental model of ischemic stroke that is considered to closely mimic embolic stroke in humans. In this model, the common carotid artery typically remains temporarily occluded to permit time for stabilization of the clot in the middle cerebral artery. However, the associated lengthening of the anesthesia duration could affect arterial blood pressure and stroke outcome. NEW

methodWe refined the model by examining how increasing isoflurane anesthesia duration from 30 to 60 min after clot embolization affects mortality, infarct volume, edema, blood-brain barrier permeability, and the 8-h post-ischemic time course of blood pressure, which has not been reported previously in this model.

resultsWe found that arterial pressure increased after discontinuing anesthesia in both embolized groups and that the increase was greater than in the corresponding non-embolized sham-operated rats. At 24 h, the group with 60-min post-ischemia anesthesia exhibited greater brain water content and a greater ipsilateral-to-contralateral ratio of extravasated Evans blue dye. Mortality was greater in the 60-min group, but infarct volume among survivors was not different from that in the 30-min anesthesia group. COMPARISON WITH EXISTING

methodsThis study refines the embolic stroke model by demonstrating the importance of minimizing the duration of anesthesia after embolization.

conclusionsThese data indicate that early discontinuation of isoflurane anesthesia after clot embolization permits an earlier hypertensive response that limits edema formation and mortality without significantly affecting infarct volume in survivors, thereby decreasing the required number of animals.

Indexed as

Disease Models, AnimalStrokeAnesthesiaAnimalsBlood-Brain BarrierBlood PressureBrain InfarctionBrain IschemiaFunctional LateralityInfarction, Middle Cerebral ArteryMaleRatsRats, WistarTime FactorsAnimal model of strokeEmbolic strokeIsoflurane anesthesiaMiddle cerebral artery occlusionRat

Identifiers

PMID29935198
PMCPMC6192029
OpenAlexW2809071400

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.