ArticleScientific reports2018
New plasma preparation approach to enrich metabolome coverage in untargeted metabolomics: plasma protein bound hydrophobic metabolite release with proteinase K.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
- Mass Spectrometry as a Tool for Metabolomics in Cancer.Advances in experimental medicine and biology · 2026Review
- Formic Acid Pretreatment Enhances Untargeted Serum and Plasma Metabolomics.Analytical chemistry · 2025Article
- Impact of myocardial revascularization surgery on the plasma metabolome.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Characterisation of the Plasma and Faecal Metabolomes in Participants with Functional Gastrointestinal Disorders.International journal of molecular sciences · 2024Article
- Multiplatform metabolomic interlaboratory study of a whole human stool candidate reference material from omnivore and vegan donors.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Enhanced molecular release from elderly bone samples using collagenase I: insights into fatty acid metabolism alterations.Journal of translational medicine · 2024Article
- NOTCH localizes to mitochondria through the TBC1D15-FIS1 interaction and is stabilized via blockade of E3 ligase and CDK8 recruitment to reprogram tumor-initiating cells.Experimental & molecular medicine · 2024Article
- Improved Screening of Monoclonal Gammopathy Patients by MALDI-TOF Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2023Article
- Plasma untargeted metabolomics with proteinase K discloses phospholipid signature associated with pulmonary arterial hypertension.Scientific reports · 2023Article
- Metabolomics Point out the Effects of Carfilzomib on Aromatic Amino Acid Biosynthesis and Degradation.International journal of molecular sciences · 2023Article
- Comparison between 5 extractions methods in either plasma or serum to determine the optimal extraction and matrix combination for human metabolomics.Cellular & molecular biology letters · 2023Article
- Comparison of the Determination of Some Antihypertensive Drugs in Clinical Human Plasma Samples by Solvent Front Position Extraction and Precipitation Modes.Molecules (Basel, Switzerland) · 2023Article
- Investigation of preclinical pharmacokinetics ofFrontiers in chemistry · 2023Article
- An Untargeted Metabolomics Approach on Carfilzomib-Induced Nephrotoxicity.Molecules (Basel, Switzerland) · 2022Article
- Characterization of the Antibacterial Activity of an SiOAntibiotics (Basel, Switzerland) · 2022Article
- Telotristat ethyl reverses myxomatous changes in mice mitral valves.Frontiers in cardiovascular medicine · 2022Article
- Reduced Growth, Altered Gut Microbiome and Metabolite Profile, and Increased Chronic Kidney Disease Risk in Young Pigs Consuming a Diet Containing Highly Resistant Protein.Frontiers in nutrition · 2022Article
- Inside the Alterations of Circulating Metabolome in Antarctica: The Adaptation to Chronic Hypoxia.Frontiers in physiology · 2022Article
- Defining Blood Plasma and Serum Metabolome by GC-MS.Metabolites · 2021Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification of novel biomarkers. The plasma proteins provide the environment for transport of hydrophobic metabolites. The current sample preparation protocol relies on the immediate precipitation of proteins and thus leads to co-precipitation of a significant fraction of hydrophobic metabolites. Here we present a new simple procedure that overcomes the co-precipitation problem and improves metabolome coverage. Introducing an additional step preceding the protein precipitation, namely limited digestion with proteinase K, allows release of associated metabolites through the relaxation of the native proteins tertiary structure. The modified protocol allows clear detection of hydrophobic metabolites including fatty acids and phospholipids. Considering the potential involvement of the hydrophobic metabolites in human cardiovascular and cancer diseases, the method may constitute a novel approach in plasma untargeted metabolomics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.