Evidence map›Paper›PMID 29934330›Full record

ArticleApplied and environmental microbiology2018

A Transposon Mutagenesis System for Bifidobacterium longum subsp. longum Based on an IS

Mikiyasu Sakanaka, Shingo Nakakawaji, Shin Nakajima, Satoru Fukiya, Arisa Abe, Wataru Saburi, Haruhide Mori, Atsushi Yokota

Open access · bronzeAbstract read
In one paragraph

Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Mikiyasu SakanakaLaboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Shingo NakakawajiLaboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Shin NakajimaLaboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Satoru FukiyaLaboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan s-fukiya@chem.agr.hokudai.ac.jp.ORCID 0000-0002-3147-1070
Arisa AbeLaboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Wataru SaburiLaboratory of Biochemistry, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Haruhide MoriLaboratory of Biochemistry, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Atsushi YokotaLaboratory of Microbial Physiology, Research Faculty of Agriculture, Hokkaido University, Sapporo, Hokkaido, Japan.
Hokkaido University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bifidobacteria are a major component of the intestinal microbiota in humans, particularly breast-fed infants. Therefore, elucidation of the mechanisms by which these bacteria colonize the intestine is desired. One approach is transposon mutagenesis, a technique currently attracting much attention because, in combination with next-generation sequencing, it enables exhaustive identification of genes that contribute to microbial fitness. We now describe a transposon mutagenesis system for

Indexed as

alpha-GlucosidasesBifidobacterium longumDNA Transposable ElementsGastrointestinal MicrobiomeGenome, BacterialHumansIntestinesIsomaltoseMutagenesisPlasmidsSequence Analysis, DNATransposasesTrehalosealpha-GlucosidasesDNA Transposable ElementsIsomaltoseisomaltuloseTransposasesTrehalosebifidobacteriafunctional genomicsinsertion sequenceintestinal colonizationmolecular geneticstransposon mutagenesis

Identifiers

PMID29934330
PMCPMC6102999
OpenAlexW2809126537

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.