Evidence map›Paper›PMID 29934301›Full record

ReviewBiochemical Society transactions2018

Fine details in complex environments: the power of cryo-electron tomography.

Joshua Hutchings, Giulia Zanetti

Erratum issuedAbstract readReview
In one paragraph

Review in Biochemical Society transactions, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Cryo-EM: A new dawn in thyroid biology.Molecular and cellular endocrinology · 2021
    Review
  7. Review
  8. Mechanisms of ligand binding.Biophysics reviews · 2020
    Review
  9. Review
  10. Article
  11. Investigating eukaryotic cells with cryo-ET.Molecular biology of the cell · 2020
    Article
  12. Article
  13. Review
  14. In situ structure determination by subtomogram averaging.Current opinion in structural biology · 2019
    Review
  15. Review
  16. Article
  17. International journal of molecular sciences · 2019
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors.

Joshua HutchingsInstitute of Structural and Molecular Biology, Birkbeck College, Malet St., London WC1E 7HX, U.K.
Giulia ZanettiInstitute of Structural and Molecular Biology, Birkbeck College, Malet St., London WC1E 7HX, U.K. g.zanetti@mail.cryst.bbk.ac.uk.

Funding

Wellcome Trust 109161/Z/15/A
6 · The paper itself

Abstract

Cryo-electron tomography (CET) is uniquely suited to obtain structural information from a wide range of biological scales, integrating and bridging knowledge from molecules to cells. In particular, CET can be used to visualise molecular structures in their native environment. Depending on the experiment, a varying degree of resolutions can be achieved, with the first near-atomic molecular structures becoming recently available. The power of CET has increased significantly in the last 5 years, in parallel with improvements in cryo-EM hardware and software that have also benefited single-particle reconstruction techniques. In this review, we cover the typical CET pipeline, starting from sample preparation, to data collection and processing, and highlight in particular the recent developments that support structural biology

Indexed as

AnimalsComputersCryoelectron MicroscopyData CollectionElectron Microscope TomographyHumansImage Processing, Computer-AssistedMolecular StructureSoftware Designcryo-electron microscopycryo-electron tomographysubtomogram averaging

Identifiers

PMID29934301
PMCPMC6103461

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.