Evidence map›Paper›PMID 29933760›Full record

ArticleJournal of neuroinflammation2018

Bone marrow-derived mesenchymal stem/stromal cells reverse the sensorial diabetic neuropathy via modulation of spinal neuroinflammatory cascades.

Afrânio Ferreira Evangelista, Marcos André Vannier-Santos, Gessica Sabrina de Assis Silva, Daniela Nascimento Silva, Paulo José Lima Juiz, Carolina Kymie Vasques Nonaka, Ricardo Ribeiro Dos Santos, Milena Botelho Pereira Soares, Cristiane Flora Villarreal

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In one paragraph

Article in Journal of neuroinflammation, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed, 3 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 3 syntheses or guidelines pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Afrânio Ferreira EvangelistaGonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZ, Salvador, BA, CEP 40296-710, Brazil.
Marcos André Vannier-SantosOswaldo Cruz Institute, Oswaldo Cruz Foundation-IOC-FIOCRUZ, Rio de Janeiro, RJ, CEP 21040-900, Brazil.
Gessica Sabrina de Assis SilvaPharmacy College, Federal University of Bahia, Salvador, BA, CEP 40170-290, Brazil.
Daniela Nascimento SilvaCenter of Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, CEP 41253-190, Brazil.
Paulo José Lima JuizFederal University of Recôncavo of Bahia, Feira de Santana, BA, CEP 44042-280, Brazil.
Carolina Kymie Vasques NonakaCenter of Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, CEP 41253-190, Brazil.
Ricardo Ribeiro Dos SantosCenter of Biotechnology and Cell Therapy, São Rafael Hospital, Salvador, BA, CEP 41253-190, Brazil.
Milena Botelho Pereira SoaresGonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZ, Salvador, BA, CEP 40296-710, Brazil.
Cristiane Flora VillarrealGonçalo Moniz Institute, Oswaldo Cruz Foundation-FIOCRUZ, Salvador, BA, CEP 40296-710, Brazil. cfv@ufba.br.

Funding

CNPq 445547/2014-6FAPESB DTE 0046/2011
6 · The paper itself

Abstract

backgroundDiabetic neuropathy (DN) is a frequent and debilitating manifestation of diabetes mellitus, to which there are no effective therapeutic approaches. Mesenchymal stem/stromal cells (MSC) have a great potential for the treatment of this syndrome, possibly through regenerative actions on peripheral nerves. Here, we evaluated the therapeutic effects of MSC on spinal neuroinflammation, as well as on ultrastructural aspects of the peripheral nerve in DN-associated sensorial dysfunction.

methodsC57Bl/6 mice were treated with bone marrow-derived MSC (1 × 10

resultsDiabetic mice presented behavioral signs of sensory neuropathy, mechanical allodynia, and heat hypoalgesia, which were completely reversed by a single administration of MSC or CM-MSC. The ultrastructural analysis of the sciatic nerve showed that diabetic mice exhibited morphological and morphometric alterations, considered hallmarks of DN, such as degenerative changes in axons and myelin sheath, and reduced area and density of unmyelinated fibers. In MSC-treated mice, these structural alterations were markedly less commonly observed and/or less pronounced. Moreover, MSC transplantation inhibited multiple parameters of spinal neuroinflammation found in diabetic mice, causing the reduction of activated astrocytes and microglia, oxidative stress signals, galectin-3, IL-1β, and TNF-α production. Conversely, MSC increased the levels of anti-inflammatory cytokines, IL-10, and TGF-β.

conclusionsThe present study described the modulatory effects of MSC on spinal cord neuroinflammation in diabetic mice, suggesting new mechanisms by which MSC can improve DN.

Indexed as

AnimalsBone Marrow TransplantationCalcium-Binding ProteinsCulture Media, ConditionedCytokinesDiabetic NeuropathiesDisease Models, AnimalGreen Fluorescent ProteinsHyperalgesiaLipid PeroxidationMaleMesenchymal Stem CellsMiceMice, Inbred C57BLMice, TransgenicMicrofilament ProteinsAif1 protein, mouseCalcium-Binding ProteinsCulture Media, ConditionedCytokinesGreen Fluorescent ProteinsMicrofilament ProteinsNitritesStreptozocinDiabetesGalectin-3NeuroinflammationSensory neuropathySpinal cordStem cells

Identifiers

PMID29933760
PMCPMC6015468

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.