ArticleMedical science monitor : international medical journal of experimental and clinical research2018
Lead Induces Genotoxicity via Oxidative Stress and Promoter Methylation of DNA Repair Genes in Human Lymphoblastoid TK6 Cells.
Article in Medical science monitor : international medical journal of experimental and clinical research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Combined Occupational Exposure to Lead and Other Metals and Risk of Lung Cancer.La Medicina del lavoro · 2026Article
- Lead Exposure Triggers DNA Damage in TK6 Cells Via Alteration of DNA Repair Gene Expression and Hypermethylation of Ku80 Promoter Region.Biological trace element research · 2026Article
- Article
- Association between blood lead and gastrointestinal cancers among US adults: A cross-sectional study based on NHANES 2003-2016.Medicine · 2025Article
- Spirulina as a natural shield: a comprehensive review of its protective effects against lead toxicity.Archives of toxicology · 2025Review
- Protective role of savory essential oil on vital organs in rats against deleterious effects induced by lead acetate.Biotechnology reports (Amsterdam, Netherlands) · 2025Article
- Tl(I) and Tl(III)-induce genotoxicity, reticulum stress and autophagy in PC12 Adh cells.Archives of toxicology · 2024Article
- Metabolic and genetic derangement: a review of mechanisms involved in arsenic and lead toxicity and genotoxicity.Arhiv za higijenu rada i toksikologiju · 2022Review
- Accumulation and Enrichment of Trace Elements by Yeast Cells and Their Applications: A Critical Review.Microorganisms · 2022Review
- Recent Approaches to Determine Static and Dynamic Redox State-Related Parameters.Antioxidants (Basel, Switzerland) · 2022Review
- Genotoxic Effects of Lead and Their Impact on the Expression of DNA Repair Genes.International journal of environmental research and public health · 2022Review
- 14-weeks combined exercise epigenetically modulated 118 genes of menopausal women with prediabetes.Frontiers in endocrinology · 2022Article
- Epimutational effects of electronic cigarettes.Environmental science and pollution research international · 2021Review
- Cognitive Impairment Induced by Lead Exposure during Lifespan: Mechanisms of Lead Neurotoxicity.Toxics · 2021Review
- Effect of lead on proliferation, oxidative stress and genotoxic damage of 3T3-L1 fibroblasts.Toxicology research · 2020Article
- Identification of the Genetic Requirements for Zinc Tolerance and Toxicity inG3 (Bethesda, Md.) · 2020Article
- Environmentally Induced Epigenetic Plasticity in Development: Epigenetic Toxicity and Epigenetic Adaptation.Current epidemiology reports · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND Lead (Pb) is a widely used metal in modern industry and is regarded as a health hazard. Although lead-induced genotoxicity has been confirmed, the direct evidence that lead induces genotoxicity in human cells and its related mechanisms has not been fully elucidated. In this study, for the first time, we evaluated the genotoxicity induced by lead in human lymphoblastoid TK6 cells. MATERIAL AND METHODS The TK6 cells were incubated with various concentrations of Pb(Ac)2 for 6 h, 12 h, or 24 h. Cell viability was detected by CCK8 assay. Various biochemical markers were assessed by specific kits. Immunofluorescence assay was used to detect g-H2AX foci formation. The promoter methylation was assessed by methylation-specific PCR. The protein levels were determined by Western blot assay. RESULTS The results showed that after exposure to lead, cell viability was obviously decreased and γ-H2AX foci formation was significantly enhanced in TK6 cells. Moreover, the levels of 8-OHdG, ROS, MDA, and GSSG were increased, while the GSH level and SOD activity were decreased in lead-treated TK6 cells. The activation of the Nrf2-ARE signaling pathway was involved in lead-induced oxidative stress in TK6 cells. Finally, the expressions of DNA repair genes XRCC1, hOGG-1, BRCA1, and XPD were inhibited via enhancing their promoter methylation in TK6 cells after exposure to lead. CONCLUSIONS Taken together, our study provides the first published evidence that lead exposure results in DNA damage via promoting oxidative stress and the promoter methylation of DNA repair genes in human lymphoblastoid TK6 cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.