Evidence map›Paper›PMID 29932890›Full record

ArticleAnalytical biochemistry2018

Expression and purification of nuclease-free protocatechuate 3,4-dioxygenase for prolonged single-molecule fluorescence imaging.

Gayan Senavirathne, Miguel A Lopez, Ryan Messer, Richard Fishel, Kristine E Yoder

Open access · hybridAbstract read
In one paragraph

Article in Analytical biochemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
1.8field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Gayan SenavirathneDepartment of Cancer Biology and Genetics, The Ohio State University Medical Center, Columbus, OH, 43210, USA.
Miguel A LopezDepartment of Cancer Biology and Genetics, The Ohio State University Medical Center, Columbus, OH, 43210, USA.
Ryan MesserDepartment of Cancer Biology and Genetics, The Ohio State University Medical Center, Columbus, OH, 43210, USA.
Richard FishelDepartment of Cancer Biology and Genetics, The Ohio State University Medical Center, Columbus, OH, 43210, USA. Electronic address: rfishel@osu.edu.
Kristine E YoderDepartment of Cancer Biology and Genetics, The Ohio State University Medical Center, Columbus, OH, 43210, USA. Electronic address: yoder.176@osu.edu.
The Ohio State University Wexner Medical Center · US

Funding

The Human Mismatch Repair Proteins and CarcinogenesisR01CA067007 · NCI · UNIVERSITY OF VERMONT &ST AGRIC COLLEGE · PI Richard Fishel · 1995 to 2026
$9.6M
Recombination/Repair Complex in Human CellsR01GM080176 · NIGMS · OHIO STATE UNIVERSITY · PI FISHEL, RICHARD · 2006 to 2015
$3.2M
Visualization of HIV-1 integration in real timeR01AI150496 · NIAID · OHIO STATE UNIVERSITY · PI KRISTINE E YODER · 2020 to 2026
$2.3M
The Mechanics of Retroviral IntegrationR01AI126742 · NIAID · OHIO STATE UNIVERSITY · PI FISHEL, RICHARD, YODER, KRISTINE E · 2017 to 2021
$1.9M
Visualization of HIV-1 integration in real timeR01GM121284 · NIGMS · OHIO STATE UNIVERSITY · PI YODER, KRISTINE E · 2017 to 2019
$1.1M
NCI NIH HHS R01 CA067007NIAID NIH HHS R01 AI126742NIAID NIH HHS R01 AI150496NIGMS NIH HHS R01 GM080176NIGMS NIH HHS R01 GM121284
6 · The paper itself

Abstract

Single-molecule (SM) microscopy is a powerful tool capable of visualizing individual molecules and events in real time. SM imaging may rely on proteins or nucleic acids labelled with a fluorophore. Unfortunately photobleaching of fluorophores leads to irreversible loss of signal, impacting the collection of data from SM experiments. Trace amounts of dissolved oxygen (O

Indexed as

Bacterial ProteinsGene ExpressionOptical ImagingProtocatechuate-3,4-DioxygenasePseudomonas putidaDeoxyribonucleasesEnzyme StabilityHydroxybenzoatesOxygenRecombinant ProteinsBacterial ProteinsDeoxyribonucleasesHydroxybenzoatesOxygenProtocatechuate-3,4-Dioxygenaseprotocatechuic acidRecombinant ProteinsNuclease contaminantsOxygen-scavenging systemsPhotobleachingProtein purificationProtocatechuate 3,4 dioxygenaseSingle-molecule fluorescence

Identifiers

PMID29932890
PMCPMC6076860
OpenAlexW2809685708

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.