Evidence map›Paper›PMID 29932798›Full record

ArticlePlant signaling & behavior2018

Improved recombinant protein production in Arabidopsis thaliana.

A von Schaewen, I S Jeong, S Rips, A Fukudome, J Tolley, Y Nagashima, K Fischer, H Kaulfuerst-Soboll, H Koiwa

Open access · bronzeAbstract read
In one paragraph

Article in Plant signaling & behavior, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Plant Platforms for Efficient Heterologous Protein Production.Biotechnology and bioprocess engineering : BBE · 2021
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 4 countries.

A von Schaewena Molekulare Physiologie der Pflanzen; Institut für Biologie & Biotechnologie der Pflanzen , Westfälische Wilhelms-Universität Münster , Münster , Germany.
I S Jeongb Vegetable and Fruit Improvement Center; Department of Horticultural Sciences; and Molecular and Environmental Plant Science Program , Texas A&M University; College Station , Texas , USA.
S Ripsa Molekulare Physiologie der Pflanzen; Institut für Biologie & Biotechnologie der Pflanzen , Westfälische Wilhelms-Universität Münster , Münster , Germany.
A Fukudomeb Vegetable and Fruit Improvement Center; Department of Horticultural Sciences; and Molecular and Environmental Plant Science Program , Texas A&M University; College Station , Texas , USA.
J Tolleyb Vegetable and Fruit Improvement Center; Department of Horticultural Sciences; and Molecular and Environmental Plant Science Program , Texas A&M University; College Station , Texas , USA.
Y Nagashimab Vegetable and Fruit Improvement Center; Department of Horticultural Sciences; and Molecular and Environmental Plant Science Program , Texas A&M University; College Station , Texas , USA.
K Fischera Molekulare Physiologie der Pflanzen; Institut für Biologie & Biotechnologie der Pflanzen , Westfälische Wilhelms-Universität Münster , Münster , Germany.
H Kaulfuerst-Sobolla Molekulare Physiologie der Pflanzen; Institut für Biologie & Biotechnologie der Pflanzen , Westfälische Wilhelms-Universität Münster , Münster , Germany.
H Koiwab Vegetable and Fruit Improvement Center; Department of Horticultural Sciences; and Molecular and Environmental Plant Science Program , Texas A&M University; College Station , Texas , USA.
University of Münster · DEGenetics and Improvement of Fruit and Vegetables · FRCatholic Kwandong University · KRCollege Station Medical Center · USTexas A&M University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Production and isolation of recombinant proteins are key steps in modern Molecular Biology. Expression vectors and platforms for various hosts, including both prokaryotic and eukaryotic systems, have been used. In basic plant research, Arabidopsis thaliana is the central model for which a wealth of genetic and genomic resources is available, and enormous knowledge has been accumulated over the past years - especially since elucidation of its genome in 2000. However, until recently an Arabidopsis platform had been lacking for preparative-scale production of homologous recombinant proteins. We recently established an Arabidopsis-based super-expression system, and used it for a structural pilot study of a multi-subunit integral membrane protein complex. This review summarizes the benefits and further potential of the model plant system for protein productions. ABBREVIATIONS: Nb, Nicotiana benthamiana; OT, oligosaccharyltransferase.

Indexed as

ArabidopsisArabidopsis ProteinsCell MembraneEndoplasmic ReticulumGolgi ApparatusRecombinant ProteinsArabidopsis ProteinsRecombinant ProteinsArabidopsiscomplex-type N-glycansEndoplasmic ReticulumGolgi apparatusplasma membraneprotein N-glycosylationrecombinant proteinstrans-Golgi network

Identifiers

PMID29932798
PMCPMC6110358
OpenAlexW2809425046

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.