ArticleBiomacromolecules2018
Ice Recrystallization Inhibiting Polymers Enable Glycerol-Free Cryopreservation of Microorganisms.
Article in Biomacromolecules, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 78 citations in OpenAlex.
- Identifying effective cryoprotection agents for non-model bacterial species.Microbiology spectrum · 2026Article
- Ice-binding protein's dual roles for prolonging the enzymatic activity of alcohol dehydrogenase against freezing.International journal of biological macromolecules · 2026Article
- Size Effect of Stem Cell Spheroids in Their Cryopreservation Using Low-Molecular-Weight PEGs as a Cryoprotectant.Biomaterials research · 2026Article
- Influence of polyethylene glycol on the mucus penetration and stability of lipid nanoparticles in cryopreservation and lyophilization.RSC advances · 2025Article
- PVAylation: precision end-functionalized poly(vinyl alcohol) for site-selective bioconjugation.Chemical science · 2025Article
- FBS-based cryoprotective compositions for effective cryopreservation of gut microbiota and key intestinal microorganisms.BMC research notes · 2024Article
- Screening of Hydrophilic Polymers Reveals Broad Activity in Protecting Phages during Cryopreservation.Biomacromolecules · 2024Article
- Low-Molecular-Weight PEGs for Cryopreservation of Stem Cell Spheroids.Biomaterials research · 2024Article
- Local solvation structures govern the mixing thermodynamics of glycerol-water solutions.Chemical science · 2023Article
- New insight into the mechanism by which antifreeze peptides regulate the physiological function of Streptococcus thermophilus subjected to freezing stress.Journal of advanced research · 2023Article
- Rediscovery of poly(ethylene glycol)s as a cryoprotectant for mesenchymal stem cells.Biomaterials research · 2023Article
- Degradable Polyampholytes from Radical Ring-Opening Copolymerization Enhance Cellular Cryopreservation.ACS macro letters · 2022Article
- Ice-binding proteins and bioinspired synthetic mimics in non-physiological environments.iScience · 2022Review
- Preparation, Characterization, and Mechanism of Antifreeze Peptides from Defatted Antarctic Krill (Molecules (Basel, Switzerland) · 2022Article
- Antifreeze Peptides Preparation from Tilapia Skin and Evaluation of Its Cryoprotective Effect onFoods (Basel, Switzerland) · 2022Article
- Red Blood Cell Cryopreservation with Minimal Post-Thaw Lysis Enabled by a Synergistic Combination of a Cryoprotecting Polyampholyte with DMSO/Trehalose.Biomacromolecules · 2022Article
- Snow flea antifreeze peptide for cryopreservation of lactic acid bacteria.NPJ science of food · 2022Article
- The role of antifreeze glycoprotein (AFGP) and polyvinyl alcohol/polyglycerol (X/Z-1000) as ice modulators during partial freezing of rat livers.Frontiers in physics · 2022Article
- Polymer-Mediated Cryopreservation of Bacteriophages.Biomacromolecules · 2021Article
- Enhanced Cellular Cryopreservation by Biopolymer-Associated Suppression of RhoA/ROCK Signaling Pathway.Materials (Basel, Switzerland) · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
All modern molecular biology and microbiology is underpinned by not only the tools to handle and manipulate microorganisms but also those to store, bank, and transport them. Glycerol is the current gold-standard cryoprotectant, but it is intrinsically toxic to most microorganisms: only a fraction of cells survive freezing and the presence of glycerol can impact downstream applications and assays. Extremophile organisms survive repeated freeze/thaw cycles by producing antifreeze proteins which are potent ice recrystallization inhibitors. Here we introduce a new concept for the storage/transport of microorganisms by using ice recrystallization inhibiting poly(vinyl alcohol) in tandem with poly(ethylene glycol). This cryopreserving formulation is shown to result in a 4-fold increase in E. coli yield post-thaw, compared to glycerol, utilizing lower concentrations, and successful cryopreservation shown as low as 1.1 wt % of additive. The mechanism of protection is demonstrated to be linked not only to inhibiting ice recrystallization (by comparison to a recombinant antifreeze protein) but also to the significantly lower toxicity of the polymers compared to glycerol. Optimized formulations are presented and shown to be broadly applicable to the cryopreservation of a panel of Gram-negative, Gram-positive, and mycobacteria strains. This represents a step-change in how microorganisms will be stored by the design of new macromolecular ice growth inhibitors; it should enable a transition from traditional solvent-based to macromolecular microbiology storage methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.