ArticleMarine biotechnology (New York, N.Y.)2018
Constitutive and Chloroplast Targeted Expression of Acetyl-CoA Carboxylase in Oleaginous Microalgae Elevates Fatty Acid Biosynthesis.
Article in Marine biotechnology (New York, N.Y.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 55 citations in OpenAlex.
- The marine diatom Phaeodactylum tricornutum as a versatile bioproduction chassis: Current progress, challenges, and perspectives.Plant communications · 2025Review
- Heat shock transcription factor-mediated thermal tolerance and cell size plasticity in marine diatoms.Nature communications · 2025Article
- Regulation of Oil Biosynthesis and Genetic Improvement in Plants: Advances and Prospects.Genes · 2024Review
- Design and assembly of the 117-kb Phaeodactylum tricornutum chloroplast genome.Plant physiology · 2024Article
- Differential protein analysis of saline-alkali promoting the oil accumulation in Nitzschia palea.Biotechnology for biofuels and bioproducts · 2024Article
- Contribution of the leaf and silique photosynthesis to the seeds yield and quality of oilseed rape (Brassica napus L.) in reproductive stage.Scientific reports · 2023Article
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- Characterization of a Novel Acyl-ACP ΔFrontiers in microbiology · 2020Article
- A novel endogenous selection marker for the diatom Phaeodactylum tricornutum based on a unique mutation in phytoene desaturase 1.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Photosynthetic microalgae are of burgeoning interest in the generation of commercial bioproducts. Microalgae accumulate high lipid content under adverse conditions, which in turn compromise their growth and hinder their commercial potential. Hence, it is necessary to engineer microalgae to mitigate elevated lipid accumulation and biomass. In this study, we identified acetyl-CoA carboxylase (ACCase) in oleaginous microalga Phaeodactylum tricornutum (PtACC2) and expressed constitutively in the chloroplast to demonstrate the potential of chloroplast engineering. Molecular characterization of transplastomic microalgae revealed that PtACC2 was integrated, transcribed and expressed successfully, and localized in the chloroplast. Enzymatic activity of ACCase was elevated by 3.3-fold, and the relative neutral lipid content increased substantially by 1.77-fold, and lipid content reached up to 40.8% of dry weight. Accordingly, the number and size of oil bodies markedly increased. Fatty acid profiling showed that the content of monounsaturated fatty acids increased, while polyunsaturated fatty acids decreased. This method provides a valuable genetic engineering toolbox for microalgal bioreactors with industrial significance.
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Registered trials
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