Evidence map›Paper›PMID 29931533›Full record

ArticleMolecular biology reports2018

Modulatory effects of bufalin, an active ingredient from toad venom on voltage-gated sodium channels.

Jie Tao, Feng Jiang, Cheng Liu, Zhirui Liu, Yudan Zhu, Jian Xu, Yiqin Ge, Kan Xu, Peihao Yin

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Immortalized Dorsal Root Ganglion Neuron Cell Lines.Frontiers in cellular neuroscience · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Jie TaoDepartment of Central Laboratory and Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. jietao_putuo@foxmail.com.ORCID http://orcid.org/0000-0002-3873-2413
Feng JiangXinhua Hospital (Chongming) Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai Chongming Xinhua Translational Medical Institute for Cancer Pain, Shanghai, China.
Cheng LiuDepartment of Central Laboratory and Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Zhirui LiuDepartment of Pharmacology, Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Yudan ZhuDepartment of Central Laboratory and Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Jian XuDepartment of Central Laboratory and Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Yiqin GeDepartment of Central Laboratory and Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Kan XuDepartment of Central Laboratory and Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. diansi9@aliyun.com.
Peihao YinDepartment of Central Laboratory and Neurosurgery, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China. yinpeihao1975@hotmail.com.
Shanghai University of Traditional Chinese Medicine · CNShanghai Jiao Tong University · CN

Funding

Innovation Fund of Putuo District Health System 17-PT-10National Natural Science Foundation of China 81603410National Science Foundation of China 81402903National Science Foundation of China 81473482Research Project of Putuo Hospital, Shanghai University of Traditional Chinese Medicine 2016208Athe "Twelfth Five Year" Key Subject (Integrated Chinese and Western Medicine) of State Administration of Traditional Medicine of China, Shanghai Municipal Commission of Health and Family Planning Fund 20134Y022
6 · The paper itself

Abstract

Chan-su (toad venom) has been used as an analgesic agent in China from ancient to modern times. Bufalin, a non-peptide toxin extracted from toad venom, is considered as one of the analgesic components. The molecular mechanism underlying the anti-nociceptive effects of bufalin remains unclear so far. In this study, we investigated the pharmacological effects of bufalin on pain-related ion channels as well as animal models through patch clamping, calcium imaging and animal behavior observation. Using the whole-cell recording, bufalin caused remarkable suppressive effect on the peak currents of Nav channels (voltage gated sodium channels, VGSCs) of dorsal root ganglion neuroblastomas (ND7-23 cell) in a dose-dependent manner. Bufalin facilitated the voltage-dependent activation and induced a negative shift on the fast inactivation of VGSCs. The recovery kinetics of VGSCs were significantly slowed and the recovery proportion were reduced after administering bufalin. However, bufalin prompted no significant effect not only on Kv4.2, Kv4.3 and BK channels heterologously expressed in HEK293T cells, but also on the capsaicin and allyl isothiocyanate induced Ca

Indexed as

Amphibian VenomsAnimalsBufanolidesChinaGanglia, SpinalHEK293 CellsHumansMaleNeuroblastomaPatch-Clamp TechniquesRatsRats, Sprague-DawleyVoltage-Gated Sodium Channel BlockersVoltage-Gated Sodium ChannelsAmphibian VenomsbufalinBufanolideschan suVoltage-Gated Sodium Channel BlockersVoltage-Gated Sodium ChannelsAnti-nociceptive effectsBufalinDose dependent inhibitionND7-23 cellVoltage-gated sodium channels

Identifiers

PMID29931533
OpenAlexW2809105119

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.