ArticleFEMS microbiology ecology2018
Investigation of viable taxa in the deep terrestrial biosphere suggests high rates of nutrient recycling.
Article in FEMS microbiology ecology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 28 citations in OpenAlex.
- A global perspective on bacterial diversity in the terrestrial deep subsurface.Microbiology (Reading, England) · 2023Pooled it
- Multi-Year Biofilm Formation on Granitic Surfaces Reveals Dynamic Microbial Communities in Fennoscandian Shield Deep Groundwaters.Microbial ecology · 2026Article
- Exploring microbial diversity using cell-size fractionated enrichment incubations from subsurface aquifers at Äspö, Sweden.Communications biology · 2026Article
- Warming Causes a Decline in Baltic Sea Coastal Sediment Microbial Abundance.Environmental microbiology · 2026Article
- Diverse microbiome functions, limited temporal variation and substantial genomic conservation within sedimentary and granite rock deep underground research laboratories.Environmental microbiome · 2024Article
- Novel candidate taxa contribute to key metabolic processes in Fennoscandian Shield deep groundwaters.ISME communications · 2024Article
- Extremophilic microbial metabolism and radioactive waste disposal.Extremophiles : life under extreme conditions · 2023Review
- Thiobacillus as a key player for biofilm formation in oligotrophic groundwaters of the Fennoscandian Shield.NPJ biofilms and microbiomes · 2023Article
- The Mystery of Piezophiles: Understudied Microorganisms from the Deep, Dark Subsurface.Microorganisms · 2023Review
- Epilithic Microbial Community Functionality in Deep Oligotrophic Continental Bedrock.Frontiers in microbiology · 2022Article
- The Fennoscandian Shield deep terrestrial virosphere suggests slow motion 'boom and burst' cycles.Communications biology · 2021Article
- Detection of Viable and Total Bacterial Community in the Pit Mud of Chinese Strong-Flavor Liquor Using Propidium Monoazide Combined With Quantitative PCR and 16S rRNA Gene Sequencing.Frontiers in microbiology · 2020Article
- Article
- Article
- Depth and Dissolved Organic Carbon Shape Microbial Communities in Surface Influenced but Not Ancient Saline Terrestrial Aquifers.Frontiers in microbiology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The deep biosphere is the largest 'bioreactor' on earth, and microbes inhabiting this biome profoundly influence global nutrient and energy cycles. An important question for deep biosphere microbiology is whether or not specific populations are viable. To address this, we used quantitative PCR and high throughput 16S rRNA gene sequencing of total and viable cells (i.e. with an intact cellular membrane) from three groundwaters with different ages and chemical constituents. There were no statistically significant differences in 16S rRNA gene abundances and microbial diversity between total and viable communities. This suggests that populations were adapted to prevailing oligotrophic conditions and that non-viable cells are rapidly degraded and recycled into new biomass. With higher concentrations of organic carbon, the modern marine and undefined mixed waters hosted a community with a larger range of predicted growth strategies than the ultra-oligotrophic old saline water. These strategies included fermentative and potentially symbiotic lifestyles by candidate phyla that typically have streamlined genomes. In contrast, the old saline waters had more 16S rRNA gene sequences in previously cultured lineages able to oxidize hydrogen and fix carbon dioxide. This matches the paradigm of a hydrogen and carbon dioxide-fed chemolithoautotrophic deep biosphere.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.