ArticleJournal of experimental botany2018
Purification, characterization, and functional analysis of a novel 6G&1-FEH mainly hydrolyzing neokestose from asparagus.
Article in Journal of experimental botany, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- A Fructan 6-Exohydrolase fromPlants (Basel, Switzerland) · 2026Article
- Variation of Fructan and Its Metabolizing Enzymes in Onions with Different Storage Characteristics.Journal of applied glycoscience · 2025Article
- Neokestose suppresses the increase in plasma glucose caused by oral administration of sucrose in a streptozotocin‑induced diabetic rat.Scientific reports · 2024Article
- Composition and Functional Properties of the Edible Spear and By-Products fromFoods (Basel, Switzerland) · 2024Article
- Metabolome and transcriptome analyses reveal changes of rapeseed in response to ABA signal during early seedling development.BMC plant biology · 2024Article
- Synbiotic microencapsulation ofJournal of food science and technology · 2023Article
- Preparation, Structural Characterisation, and Bioactivities of Fructans: A Review.Molecules (Basel, Switzerland) · 2023Review
- Different evolutionary pathways to generate plant fructan exohydrolases.Journal of experimental botany · 2022Article
- A Fructan Exohydrolase from Maize Degrades Both Inulin and Levan and Co-Exists with 1-Kestotriose in Maize.International journal of molecular sciences · 2021Article
- Fructan Structure and Metabolism in Overwintering Plants.Plants (Basel, Switzerland) · 2021Review
- Article
- Mechanism of Allium Crops Bulb Enlargement in Response to Photoperiod: A Review.International journal of molecular sciences · 2020Review
- A 6&1-FEH Encodes an Enzyme for Fructan Degradation and Interact with Invertase Inhibitor Protein in Maize (International journal of molecular sciences · 2019Article
- Novel fructan exohydrolase: unique properties and applications for human health.Journal of experimental botany · 2018Article
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Asparagus (Asparagus officinalis L.) accumulates inulin- and inulin neoseries-type fructans. Fructose released by the hydrolysis of fructans is an energy source for emerging asparagus spears. Plant fructans are hydrolyzed by fructan exohydrolases (FEHs), whose presence in asparagus has not yet been fully characterized. Here, we describe for the first time the purification and characterization of an FEH from asparagus, and the functional analysis of its gene. The purified enzyme was predicted to exist as a dimer (approximately 130 kDa) consisting of two polypeptides with a molecular mass of approximately 68 kDa. N-terminal sequences of the purified enzyme were matched with the amino acid sequences of aoeh4a and aoeh4b cDNAs isolated from asparagus (cv. Gijnlim and Taihouwase). Native enzymes obtained from asparagus roots and recombinant enzymes produced by Pichia pastoris showed fructan 1-exohydrolase (1-FEH) activity via the hydrolysis of inulin-type fructan. Unlike other 1-FEHs, these enzymes showed minimal hydrolysis of 1-kestose but efficiently hydrolyzed neokestose. Therefore, the enzyme was termed 6G&1-FEH. Gene expression studies in asparagus roots showed that aoeh4 increased during root storage at 2 °C and spear harvesting. These findings suggest that 6G&1-FEH may be involved in fructan hydrolysis in asparagus roots to provide an energy source for emerging asparagus spears.
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