ArticleCellular and molecular gastroenterology and hepatology2018
Human Fetal-Derived Enterospheres Provide Insights on Intestinal Development and a Novel Model to Study Necrotizing Enterocolitis (NEC).
Article in Cellular and molecular gastroenterology and hepatology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers, 1 of them a synthesis that pooled it.
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Who cites it
57 citing papers in PubMed, 1 synthesis or guideline pooled it, 79 citations in OpenAlex.
- Enteral Feeding Interventions in the Prevention of Necrotizing Enterocolitis: A Systematic Review of Experimental and Clinical Studies.Nutrients · 2021Pooled it
- Temperature and developmental stage govern intestinal susceptibility to human coronavirus 229E.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Small intestine microbiota development prevents early-life adiposity via IL-22-mediated intestinal PPARα suppression.bioRxiv : the preprint server for biology · 2026Article
- Bridging the gap: organotypic models to study late-onset group B streptococcus infection.Microbiology spectrum · 2026Article
- Digesting the idea of extensive protein hydrolysate in support of immature intestinal barrier function.Pediatric research · 2026Article
- Effects of specialized proresolving mediators on gut epithelial barrier in early life.The Journal of pharmacology and experimental therapeutics · 2026Article
- Effects of extensive protein hydrolysate in supporting intestinal barrier function in vitro.Pediatric research · 2026Article
- Optimising the induction of inflammation within preterm infant-derived intestinal epithelial organoids.Npj gut and liver · 2026Article
- Synergism between TLR4 and B. infantis in the development of the premature intestine.Pediatric research · 2025Article
- Neutrophils aggravate inflammatory lesions in intestinal organoids from necrotizing enterocolitis.Frontiers in immunology · 2025Article
- Development of aApplied and environmental microbiology · 2024Article
- Establishment and Characterization of Patient-Derived Intestinal Organoids from Pediatric Crohn's Disease Patients.Pediatric gastroenterology, hepatology & nutrition · 2024Article
- CSF1 is expressed by the intestinal epithelial cells to regulate Mφ macrophages and maintain epithelial homeostasis and is downregulated in neonates with necrotizing enterocolitis.BMC pediatrics · 2024Article
- Infant and adult human intestinal enteroids are morphologically and functionally distinct.mBio · 2024Article
- Review
- Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier.iScience · 2024Article
- Translocation across a human enteroid monolayer by zoonoticiScience · 2024Article
- Infant and Adult Human Intestinal Enteroids are Morphologically and Functionally Distinct.bioRxiv : the preprint server for biology · 2024Article
- Research Models to Mimic Necrotizing Enterocolitis and Inflammatory Bowel Diseases: Focus on Extracellular Vesicles Action.Stem cells (Dayton, Ohio) · 2023Review
- A foundational approach to culture and analyze malnourished organoids.Gut microbes · 2023Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
BACKGROUND &
aimsUntreated necrotizing enterocolitis (NEC) can lead to massive inflammation resulting in intestinal necrosis with a high mortality rate in preterm infants. Limited access to human samples and relevant experimental models have hampered progress in NEC pathogenesis. Earlier evidence has suggested that bacterial colonization of an immature and developing intestine can lead to an abnormally high inflammatory response to bacterial bioproducts. The aim of our study was to use human fetal organoids to gain insights into NEC pathogenesis.
methodsRNA sequencing analysis was performed to compare patterns of gene expression in human fetal-derived enterospheres (FEnS) and adult-derived enterospheres (AEnS). Differentially expressed genes were analyzed using computational techniques for dimensional reduction, clustering, and gene set enrichment. Unsupervised cluster analysis, Gene Ontology, and gene pathway analysis were used to predict differences between gene expression of samples. Cell monolayers derived from FEnS and AEnS were evaluated for epithelium function and responsiveness to lipopolysaccharide and commensal bacteria.
resultsBased on gene expression patterns, FEnS clustered according to their developmental age in 2 distinct groups: early and late FEnS, with the latter more closely resembling AEnS. Genes involved in maturation, gut barrier function, and innate immunity were responsible for these differences. FEnS-derived monolayers exposed to either lipopolysaccharide or commensal
conclusionsOur results provide insights into processes underlying human intestinal development and support the use of FEnS as a relevant human preclinical model for NEC. Accession number of repository for expression data: GSE101531.
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Registered trials
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