ArticleZoological letters2018
Aqueous-based tissue clearing in crustaceans.
Article in Zoological letters, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3D.Cell reports methods · 2026Article
- Progress on three-dimensional visualizing skin architecture with multiple immunofluorescence staining and tissue-clearing approaches.Histology and histopathology · 2025Review
- See-Star: a versatile hydrogel-based protocol for clearing large, opaque and calcified marine invertebrates.EvoDevo · 2024Article
- CRISPR-clear imaging of melanin-rich B16-derived solid tumors.Communications biology · 2023Article
- MAX: a simple, affordable, and rapid tissue clearing reagent for 3D imaging of wide variety of biological specimens.Scientific reports · 2022Article
- Tissue clearing and 3D imaging in developmental biology.Development (Cambridge, England) · 2021Review
- Physical and chemical mechanisms of tissue optical clearing.iScience · 2021Review
- TISSUE CLEARING.Nature reviews. Methods primers · 2021Article
- Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures.Scientific reports · 2020Article
- A versatile depigmentation, clearing, and labeling method for exploring nervous system diversity.Science advances · 2020Article
- Advanced CUBIC tissue clearing for whole-organ cell profiling.Nature protocols · 2019Article
- See-through observation of malaria parasite behaviors in the mosquito vector.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
2 authors.
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Abstract
backgroundInvestigation of the internal tissues and organs of a macroscopic organism usually requires destructive processes, such as dissection or sectioning. These processes are inevitably associated with the loss of some spatial information. Recently, aqueous-based tissue clearing techniques, which allow whole-organ or even whole-body clearing of small rodents, have been developed and opened a new method of three-dimensional histology. It is expected that these techniques will be useful tools in the field of zoology, in which organisms with highly diverse morphology are investigated and compared. However, most of these new methods are optimized for soft, non-pigmented organs in small rodents, especially the brain, and their applicability to non-model organisms with hard exoskeletons and stronger pigmentation has not been tested.
resultsWe explored the possible application of an aqueous-based tissue clearing technique, advanced CUBIC, on small crustaceans. The original CUBIC procedure did not clear the terrestrial isopod,
conclusionsIn this study, we developed a method to clear whole tissues of crustaceans. To the best of our knowledge, this is the first report of whole-mount clearing applied to crustaceans using an aqueous-based technique. This technique could facilitate morphological studies of crustaceans and other organisms with calcified exoskeletons and pigmentation.
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