ArticleScientific reports2018
A convenient protocol for generating giant unilamellar vesicles containing SNARE proteins using electroformation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- A practical guide to investigating biomolecular condensates: a comment from the plant community.Science China. Life sciences · 2026Review
- Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy.Nature communications · 2026Article
- Membrane-Associated Biomolecules for Synthetic Cell Signalling.Chembiochem : a European journal of chemical biology · 2026Review
- Combining Nanoscale Curvature and Polymer Osmotic Pressure for Efficient Giant Vesicle Assembly Under Physiological Conditions.Small methods · 2025Article
- Observing biological spatio-angular structures and dynamics with statistical image reconstruction and polarized fluorescence microscopy.bioRxiv : the preprint server for biology · 2025Article
- The Hidden Cost of Fusion: Intrinsic Asymmetry in Vesicle Fusion Restricts Synthetic Cell Growth.bioRxiv : the preprint server for biology · 2025Article
- Uncovering the Mechanisms of Intracellular Membrane Trafficking by Reconstituted Membrane Systems.Membranes · 2025Review
- Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading.Science advances · 2025Article
- On-the-Fly Microfluidic Control of Giant Vesicle Compositions Validated by DNA Surface Charge Sensors.ACS nano · 2025Article
- Research Progress of Phospholipid Vesicles in Biological Field.Biomolecules · 2024Review
- Article
- Several common methods of making vesicles (except an emulsion method) capture intended lipid ratios.Biophysical journal · 2024Article
- Triacylglycerol-droplet-induced bilayer spontaneous curvature in giant unilamellar vesicles.Biophysical journal · 2024Article
- Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films with a Focus on Vesicles with High Cholesterol Content.Membranes · 2024Article
- Protein-Rich Rafts in Hybrid Polymer/Lipid Giant Unilamellar Vesicles.Biomacromolecules · 2024Article
- Microfluidics-mediated Liposomal Nanoparticles for Cancer Therapy: Recent Developments on Advanced Devices and Technologies.Current topics in medicinal chemistry · 2024Review
- Electroformation of Giant Unilamellar Vesicles from Damp Lipid Films Formed by Vesicle Fusion.Membranes · 2023Article
- Bending Rigidity, Capacitance, and Shear Viscosity of Giant Vesicle Membranes Prepared by Spontaneous Swelling, Electroformation, Gel-Assisted, and Phase Transfer Methods: A Comparative Study.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Experimental platform for the functional investigation of membrane proteins in giant unilamellar vesicles.Soft matter · 2022Article
- Article
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3 authors.
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Abstract
Reconstitution of membrane proteins in artificial membranes is an essential prerequisite for functional studies that depend on the context of an intact membrane. While straight-forward protocols for reconstituting proteins in small unilamellar vesicles were developed many years ago, it is much more difficult to prepare large membranes containing membrane proteins at biologically relevant concentrations. Giant unilamellar vesicles (GUVs) represent a model system that is characterised by low curvature, controllable tension, and large surface that can be easily visualised with microscopy, but protein insertion is notoriously difficult. Here we describe a convenient method for efficient generation of GUVs containing functionally active SNARE proteins that govern exocytosis of synaptic vesicles. Preparation of proteo-GUVs requires a simple, in-house-built device, standard and inexpensive electronic equipment, and employs a straight-forward protocol that largely avoids damage of the proteins. The procedure allows upscaling and multiplexing, thus providing a platform for establishing and optimizing preparation of GUVs containing membrane proteins for a diverse array of applications.
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