Evidence map›Paper›PMID 29930324›Full record

ArticleScientific reports2018

Characterization of a community-acquired-MRSA USA300 isolate from a river sample in Austria and whole genome sequence based comparison to a diverse collection of USA300 isolates.

Sarah Lepuschitz, Steliana Huhulescu, Patrick Hyden, Burkhard Springer, Thomas Rattei, Franz Allerberger, Robert L Mach, Werner Ruppitsch

Abstract read
In one paragraph

Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Complete genome sequence of three methicillin-resistantMicrobiology resource announcements · 2025
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  10. The First Bacterial Endocarditis Due toPathogens (Basel, Switzerland) · 2021
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  11. The First Report ofAntibiotics (Basel, Switzerland) · 2021
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  12. Article
  13. Presence of β-Lactamase-producing Enterobacterales andInternational journal of molecular sciences · 2021
    Article
  14. Broad-Spectrum Cephalosporin-ResistantAnimals : an open access journal from MDPI · 2020
    Article
  15. Review
  16. Article
  17. Article
  18. Urban brown rats (Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2019
    Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Sarah LepuschitzAustrian Agency for Health and Food Safety, National Reference Laboratory for Coagulase Positive Staphylococci including Staphylococcus aureus, Graz, Austria. sarah.lepuschitz@ages.at.
Steliana HuhulescuAustrian Agency for Health and Food Safety, National Reference Laboratory for Coagulase Positive Staphylococci including Staphylococcus aureus, Graz, Austria.
Patrick HydenUniversity of Vienna, Department of Microbiology and Ecosystem Science, Vienna, Austria.
Burkhard SpringerAustrian Agency for Health and Food Safety, National Reference Laboratory for Coagulase Positive Staphylococci including Staphylococcus aureus, Graz, Austria.
Thomas RatteiUniversity of Vienna, Department of Microbiology and Ecosystem Science, Vienna, Austria.ORCID 0000-0002-0592-7791
Franz AllerbergerAustrian Agency for Health and Food Safety, National Reference Laboratory for Coagulase Positive Staphylococci including Staphylococcus aureus, Graz, Austria.
Robert L MachVienna University of Technology, Research Area of Biochemical Technology, Institute of Chemical, Biological and Environmental Engineering, Vienna, Austria.
Werner RuppitschAustrian Agency for Health and Food Safety, National Reference Laboratory for Coagulase Positive Staphylococci including Staphylococcus aureus, Graz, Austria.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The increasing emergence of multi-resistant bacteria in healthcare settings, in the community and in the environment represents a major health threat worldwide. In 2016, we started a pilot project to investigate antimicrobial resistance in surface water. Bacteria were enriched, cultivated on selective chromogenic media and species identification was carried out by MALDI-TOF analysis. From a river in southern Austria a methicillin resistant Staphylococcus aureus (MRSA) was isolated. Whole genome sequence analysis identified the isolate as ST8, spa type t008, SCCmecIV, PVL and ACME positive, which are main features of CA-MRSA USA300. Whole genome based cgMLST of the water isolate and comparison to 18 clinical MRSA USA300 isolates from the Austrian national reference laboratory for coagulase positive staphylococci originating from 2004, 2005 and 2016 and sequences of 146 USA300 isolates arbitrarily retrieved from the Sequence Read Archive revealed a close relatedness to a clinical isolate from Austria. The presence of a CA-MRSA USA300 isolate in an aquatic environment might pose a public health risk by serving as a potential source of infection or a source for emergence of new pathogenic MRSA clones.

Indexed as

Genome, BacterialSequence Homology, Nucleic AcidAustriaCommunity-Acquired InfectionsMethicillin-Resistant Staphylococcus aureusRivers

Identifiers

PMID29930324
PMCPMC6013426

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.