ArticleeLife2018
Computational design of thermostabilizing point mutations for G protein-coupled receptors.
Article in eLife, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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Who cites it
35 citing papers in PubMed.
- Structural insights into the high basal activity and inverse agonism of the orphan receptor GPR6 implicated in Parkinson's disease.Science signaling · 2024Article
- One-shot design elevates functional expression levels of a voltage-gated potassium channel.Protein science : a publication of the Protein Society · 2024Article
- Molecular mechanism of biased signaling at the kappa opioid receptor.Nature communications · 2023Article
- The activation mechanism and antibody binding mode for orphan GPR20.Cell discovery · 2023Article
- Stabilization ofMembranes · 2023Article
- Cryo-EM structures of orphan GPR21 signaling complexes.Nature communications · 2023Article
- Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- eGFP as an All-in-One Tag for Purification of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Beyond sequence: Structure-based machine learning.Computational and structural biotechnology journal · 2023Review
- Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP.Nature protocols · 2022Review
- Dawn of a New Era for Membrane Protein Design.Biodesign research · 2022Review
- State-Targeting Stabilization of Adenosine AInternational journal of molecular sciences · 2021Article
- Molecular basis for kinin selectivity and activation of the human bradykinin receptors.Nature structural & molecular biology · 2021Article
- Protocol for crystal structure determination of the antagonist-bound human cannabinoid receptor CB2.STAR protocols · 2021Article
- Structural insights on ligand recognition at the human leukotriene B4 receptor 1.Nature communications · 2021Article
- A novel high-throughput screen for identifying lipids that stabilise membrane proteins in detergent based solution.PloS one · 2021Article
- MutTMPredictor: Robust and accurate cascade XGBoost classifier for prediction of mutations in transmembrane proteins.Computational and structural biotechnology journal · 2021Article
- Purinergic Signaling: Impact of GPCR Structures on Rational Drug Design.ChemMedChem · 2020Review
- IMPROvER: the Integral Membrane Protein Stability Selector.Scientific reports · 2020Article
- Characterizing Interhelical Interactions of G-Protein Coupled Receptors with the Fragment Molecular Orbital Method.Journal of chemical theory and computation · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Engineering of GPCR constructs with improved thermostability is a key for successful structural and biochemical studies of this transmembrane protein family, targeted by 40% of all therapeutic drugs. Here we introduce a comprehensive computational approach to effective prediction of stabilizing mutations in GPCRs, named CompoMug, which employs sequence-based analysis, structural information, and a derived machine learning predictor. Tested experimentally on the serotonin 5-HT
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.