Evidence map›Paper›PMID 29927115›Full record

ArticleCurrent protocols in chemical biology2018

Characterizing Protein Kinase Substrate Specificity Using the Proteomic Peptide Library (ProPeL) Approach.

Joshua M Lubner, Jeremy L Balsbaugh, George M Church, Michael F Chou, Daniel Schwartz

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Current protocols in chemical biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.9field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. The Okur-Chung Neurodevelopmental Syndrome Mutation CK2Frontiers in molecular biosciences · 2022
    Article
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Joshua M LubnerUniversity of Connecticut, Department of Physiology and Neurobiology, Storrs, Connecticut.
Jeremy L BalsbaughUniversity of Connecticut, Proteomics & Metabolomics Facility, Center for Open Research Resources & Equipment, Storrs, Connecticut.
George M ChurchHarvard Medical School, Department of Genetics, Boston, Massachusetts and Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts.
Michael F ChouHarvard Medical School, Department of Genetics, Boston, Massachusetts and Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts.
Daniel SchwartzUniversity of Connecticut, Department of Physiology and Neurobiology, Storrs, Connecticut.
University of Connecticut · USBaim Institute for Clinical Research · USHarvard University · US

Funding

Determining the Substrate Specificity of Wild-Type and Encephalopathy-Associated Mutants of Protein Kinase CDKL5R21NS096516 · NINDS · UNIVERSITY OF CONNECTICUT STORRS · PI SCHWARTZ, DANIEL · 2016 to 2017
$425k
NINDS NIH HHS R21 NS096516
6 · The paper itself

Abstract

Characterizing protein kinase substrate specificity motifs represents a powerful step in elucidating kinase-signaling cascades. The protocol described here uses a bacterial system to evaluate kinase specificity motifs in vivo, without the need for radioactive ATP. The human kinase of interest is cloned into a heterologous bacterial expression vector and allowed to phosphorylate E. coli proteins in vivo, consistent with its endogenous substrate preferences. The cells are lysed, and the bacterial proteins are digested into peptides and phosphoenriched using bulk TiO

Indexed as

Peptide LibraryProteomicsHumansProtein KinasesStructure-Activity RelationshipSubstrate SpecificityPeptide LibraryProtein Kinaseskinase specificitymass spectrometryphosphorylation motifpLogoProPeLprotein kinaseproteomic peptide library

Identifiers

PMID29927115
PMCPMC6014603
OpenAlexW2809032971

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.