ArticleCurrent protocols in microbiology2018
Human Intestinal Enteroids for the Study of Bacterial Adherence, Invasion, and Translocation.
Article in Current protocols in microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- Article
- Drivers of transcriptional variance in human intestinal epithelial organoids.Physiological genomics · 2021Article
- Effect of substrate stiffness on human intestinal enteroids' infectivity by enteroaggregative Escherichia coli.Acta biomaterialia · 2021Article
- Article
- Article
- Co-Culture System of Human Enteroids/Colonoids with Innate Immune Cells.Current protocols in immunology · 2020Article
- Enteroaggregative E. coli Adherence to Human Heparan Sulfate Proteoglycans Drives Segment and Host Specific Responses to Infection.PLoS pathogens · 2020Article
- Redefining enteroaggregative Escherichia coli (EAEC): Genomic characterization of epidemiological EAEC strains.PLoS neglected tropical diseases · 2020Article
- A Comparison of Sterilization Techniques for Production of Decellularized Intestine in Mice.Tissue engineering. Part C, Methods · 2020Article
- Human Intestinal Enteroids as a Model System ofInfection and immunity · 2019Article
- Pathogenesis ofFrontiers in immunology · 2018Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Adherence, invasion, and translocation to and through the intestinal epithelium are important drivers of disease for many enteric bacteria. However, most work has been limited to transformed intestinal cell lines or murine models that often do not faithfully recapitulate key elements associated with human disease. The recent technological advances in organotypic tissue and cell culture are providing unparalleled access to systems with human physiology and complexity. Human intestinal enteroids (HIEs), derived from patient biopsy or surgical specimens of intestinal tissues, are organotypic cultures now being adapted to the study of enteric infections. HIEs are comprised of the dominant cell types of the human gastrointestinal epithelium, can be grown in two- or three-dimensional structures, form a crypt-villus axis with defined apical and basolateral compartments, and undergo physiologic responses to many different stimuli. Here, we describe a series of protocols that encompass the use of human enteroids for the measurement of the adherence, invasion, and translocation of E. coli to and through the intestinal epithelium. We also outline the steps needed to grow and prepare enteroids for this purpose and highlight some common problems to troubleshoot. © 2018 by John Wiley & Sons, Inc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.