ArticleScientific reports2018
ERK1/2 and p38 regulate inter-individual variability in ozone-mediated IL-8 gene expression in primary human bronchial epithelial cells.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 28 citations in OpenAlex.
- In vitro approaches to assess respiratory toxicity from volatile organic compounds: an in-depth review.Toxicological sciences : an official journal of the Society of Toxicology · 2026Review
- Host-specific bacterial modulation of airway gene expression and alternative splicing.mSphere · 2025Article
- Culture media influences primary human bronchial epithelial cell morphology, differentiation status, and transcriptional response to ozone exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- Anti-Inflammatory Effect of Ethanol Extract fromNutrients · 2024Article
- Epithelial MAPK signaling directs endothelial NRF2 signaling and IL-8 secretion in a tri-culture model of the alveolar-microvascular interface following diesel exhaust particulate (DEP) exposure.Particle and fibre toxicology · 2024Article
- Long Interspersed Nuclear Element-1 Analytes in Extracellular Vesicles as Tools for Molecular Diagnostics of Non-Small Cell Lung Cancer.International journal of molecular sciences · 2024Article
- New approach methodologies (NAMs) for theFrontiers in toxicology · 2024Review
- Liquid application dosing alters the physiology of air-liquid interface (ALI) primary human bronchial epithelial cell/lung fibroblast co-cultures andFrontiers in toxicology · 2023Article
- Downregulation of IL-8 and IL-10 by the Activation of CaInternational journal of molecular sciences · 2022Article
- LINE-1 Cargo and Reverse Transcriptase Activity Profiles in Extracellular Vesicles from Lung Cancer Cells and Human Plasma.International journal of molecular sciences · 2022Article
- Ozone Responsive Gene Expression as a Model for Describing Repeat Exposure Response Trajectories and Interindividual Toxicodynamic Variability In Vitro.Toxicological sciences : an official journal of the Society of Toxicology · 2021Article
- Knockdown of long non-coding RNA NEAT1 relieves the inflammatory response of spinal cord injury through targeting miR-211-5p/MAPK1 axis.Bioengineered · 2021Article
- Role of Innate Immune System in Environmental Lung Diseases.Current allergy and asthma reports · 2021Review
- Neutrophil extracellular traps activate IL-8 and IL-1 expression in human bronchial epithelia.American journal of physiology. Lung cellular and molecular physiology · 2020Article
- Comparison of the basic morphology and function of 3D lung epithelial cultures derived from several donors.Current research in toxicology · 2020Article
- Prenatal Ozone Exposure Induces Memory Deficiencies in Newborns Rats.Frontiers in molecular neuroscience · 2019Article
- Dual Role For A MEK Inhibitor As A Modulator Of Inflammation And Host Defense Mechanisms With Potential Therapeutic Application In COPD.International journal of chronic obstructive pulmonary disease · 2019Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Inter-individual variability is observed in all biological responses; however this variability is difficult to model and its underlying mechanisms are often poorly understood. This issue currently impedes understanding the health effects of the air pollutant ozone. Ozone produces pulmonary inflammation that is highly variable between individuals; but reproducible within a single individual, indicating undefined susceptibility factors. Studying inter-individual variability is difficult with common experimental models, thus we used primary human bronchial epithelial cells (phBECs) collected from many different donors. These cells were cultured, exposed to ozone, and the gene expression of the pro-inflammatory cytokine IL-8 was measured. Similar to in vivo observations, we found that ozone-mediated IL-8 expression was variable between donors, but reproducible within a given donor. Recent evidence suggests that the MAP kinases ERK1/2 and p38 mediate ozone-induced IL-8 transcription, thus we hypothesized that differences in their activation may control IL-8 inter-individual variability. We observed a significant correlation between ERK1/2 phosphorylation and IL-8 expression, suggesting that ERK1/2 modulates the ozone-mediated IL-8 response; however, we found that simultaneous inhibition of both kinases was required to achieve the greatest IL-8 inhibition. We proposed a "dimmer switch" model to explain how the coordinate activity of these kinases regulate differential IL-8 induction.
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