Evidence map›Paper›PMID 29925007›Full record

ArticleCell reports2018

MIN1PIPE: A Miniscope 1-Photon-Based Calcium Imaging Signal Extraction Pipeline.

Jinghao Lu, Chunyuan Li, Jonnathan Singh-Alvarado, Zhe Charles Zhou, Flavio Fröhlich, Richard Mooney, Fan Wang

Abstract read
In one paragraph

Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.

0numbers the graph read from it
0cells of the map it votes in
74citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

74 citing papers in PubMed.

  1. Article
  2. Stars2Cells: Astrometric Tracking of Neurons Across Imaging Sessions.bioRxiv : the preprint server for biology · 2026
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  6. Review
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  16. Article
  17. Distinct Functional Classes of CA1 Hippocampal Interneurons Are Modulated by Cerebellar Stimulation in a Coordinated Manner.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024
    Article
  18. Article
  19. Article
  20. Article

14 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jinghao LuDepartment of Neurobiology, Duke University Medical Center, Duke University, Durham, NC 27710, USA. Electronic address: jinghao.lu@duke.edu.
Chunyuan LiDepartment of Electrical and Computer Engineering, Duke University, Durham, NC 27710, USA. Electronic address: chunyuan.li@duke.edu.
Jonnathan Singh-AlvaradoDepartment of Neurobiology, Duke University Medical Center, Duke University, Durham, NC 27710, USA.
Zhe Charles ZhouDepartment of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Neurobiology Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Flavio FröhlichDepartment of Psychiatry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA; Neurobiology Curriculum, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Richard MooneyDepartment of Neurobiology, Duke University Medical Center, Duke University, Durham, NC 27710, USA.
Fan WangDepartment of Neurobiology, Duke University Medical Center, Duke University, Durham, NC 27710, USA; Department of Cell Biology, Duke University Medical Center, Duke University, Durham, NC 27710, USA. Electronic address: fan.wang@duke.edu.

Funding

Mechanisms for internally and externally guided sensorimotor learningR01NS099288 · NINDS · DUKE UNIVERSITY · PI MOONEY, RICHARD D · 2016 to 2025
$3.9M
Toward causal neuroscience: capture and manipulate emergent neuronal ensemblesDP1MH103908 · NIMH · DUKE UNIVERSITY · PI WANG, FAN · 2013 to 2017
$3.9M
Motor Modulation of Auditory ProcessingR01DC013826 · NIDCD · DUKE UNIVERSITY · PI MOONEY, RICHARD D · 2015 to 2025
$3.8M
Pre-motor Neural Circuits for Exploratory MovementR01NS077986 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI WANG, FAN · 2013 to 2022
$3.4M
Functions of distinct trigeminal brainstem projection pathways in tactile behaviorR21NS101441 · NINDS · DUKE UNIVERSITY · PI WANG, FAN · 2017 to 2018
$410k
NIDCD NIH HHS R01 DC013826NIMH NIH HHS DP1 MH103908NINDS NIH HHS R01 NS077986NINDS NIH HHS R01 NS099288NINDS NIH HHS R21 NS101441
6 · The paper itself

Abstract

In vivo calcium imaging using a 1-photon-based miniscope and a microendoscopic lens enables studies of neural activities in freely behaving animals. However, the high and fluctuating background, the inevitable movements and distortions of imaging field, and the extensive spatial overlaps of fluorescent signals emitted from imaged neurons inherent in this 1-photon imaging method present major challenges for extracting neuronal signals reliably and automatically from the raw imaging data. Here, we develop a unifying algorithm called the miniscope 1-photon imaging pipeline (MIN1PIPE), which contains several stand-alone modules and can handle a wide range of imaging conditions and qualities with minimal parameter tuning and automatically and accurately isolate spatially localized neural signals. We have quantitatively compared MIN1PIPE with other existing partial methods using both synthetic and real datasets obtained from different animal models and show that MIN1PIPE has superior efficiency and precision in analyzing noisy miniscope calcium imaging data.

Indexed as

Imaging, Three-DimensionalPhotonsSignal Processing, Computer-AssistedSoftwareAnimalsCalciumDatabases as TopicFinchesMiceReproducibility of ResultsVocalization, AnimalCalciumautomatic signal extractioncalcium imagingminiscopemovement correctionsingle-photon

Identifiers

PMID29925007
PMCPMC6084484

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.