ArticleCurrent protocols in cell biology2018
Multispectral Live-Cell Imaging.
Article in Current protocols in cell biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Deep unfolding blind source unmixing for multicolor fluorescence imaging.Biomedical optics express · 2026Article
- Resolving fluorescently labeled species using highly multiplexed spectral FLIM.Scientific reports · 2026Article
- Neurons and astrocytes have distinct organelle signatures and responses to stress.Cell reports · 2025Article
- Doubling multiplexed imaging capability via spatial expression pattern-guided protein pairing and computational unmixing.Communications biology · 2025Article
- Bright and photostable yellow fluorescent proteins for extended imaging.Nature communications · 2025Article
- Imaging interorganelle contacts at a glance.Journal of cell science · 2024Review
- Multispectral imaging for characterizing autofluorescent tissues.Scientific reports · 2024Article
- In Vitro Dental Plaque Culture Model for Biofilm Structural Analyses.Current protocols · 2023Article
- PICASSO allows ultra-multiplexed fluorescence imaging of spatially overlapping proteins without reference spectra measurements.Nature communications · 2022Article
- Soft X-ray tomography to map and quantify organelle interactions at the mesoscale.Structure (London, England : 1993) · 2022Article
- Live-cell fluorescence spectral imaging as a data science challenge.Biophysical reviews · 2022Review
- An intensity-based post-processing tool for 3D instance segmentation of organelles in soft X-ray tomograms.PloS one · 2022Article
- Multispectral Image under Tissue Classification Algorithm in Screening of Cervical Cancer.Journal of healthcare engineering · 2022Article
- Spectral imaging and nucleic acid mimics fluorescenceFrontiers in microbiology · 2022Article
- Review
- Phasor-based hyperspectral snapshot microscopy allows fast imaging of live, three-dimensional tissues for biomedical applications.Communications biology · 2021Article
- Advances in Confocal Microscopy and Selected Applications.Methods in molecular biology (Clifton, N.J.) · 2021Review
- Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging.iScience · 2020Review
- Integrated Neurophotonics: Toward Dense Volumetric Interrogation of Brain Circuit Activity-at Depth and in Real Time.Neuron · 2020Review
- Detection of LC3-Associated Phagocytosis (LAP).Current protocols in cell biology · 2020Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
Abstract
Fluorescent proteins and vital dyes are invaluable tools for studying dynamic processes within living cells. However, the ability to distinguish more than a few different fluorescent reporters in a single sample is limited by the spectral overlap of available fluorophores. Here, we present a protocol for imaging live cells labeled with six fluorophores simultaneously. A confocal microscope with a spectral detector is used to acquire images, and linear unmixing algorithms are applied to identify the fluorophores present in each pixel of the image. We describe the application of this method to visualize the dynamics of six different organelles, and to quantify the contacts between organelles. However, this method can be used to image any molecule amenable to tagging with a fluorescent probe. Thus, multispectral live-cell imaging is a powerful tool for systems-level analysis of cellular organization and dynamics. © 2018 by John Wiley & Sons, Inc.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.