Evidence map›Paper›PMID 29923069›Full record

ArticleMolecular genetics and genomics : MGG2018

Construction of a highly efficient display system for baculovirus and its application on multigene co-display.

Hao Zheng, Xiong Wang, Feifei Ren, Shenglong Zou, Min Feng, Liangliang Xu, Lunguang Yao, Jingchen Sun

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular genetics and genomics : MGG, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Hao ZhengGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding and Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Xiong WangGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding and Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Feifei RenGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding and Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Shenglong ZouGuangzhou Cynosure Bio-Technology Co., Ltd, Guangzhou, 510080, Guangdong, China.
Min FengGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding and Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Liangliang XuGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding and Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China.
Lunguang YaoChina-UK NYNU-RRes Joint Lab of Insect Biology and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-Line of South-to-North Diversion Project, Collaborative Innovation Center of Water Security for Water Source Region of Mid-Line of South-to-North Diversion Project of Henan Province, Nanyang Normal University, Nanyang, 473061, Henan, China. lunguangyao@163.com.
Jingchen SunGuangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding and Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, College of Animal Science, South China Agricultural University, Guangzhou, 510642, Guangdong, China. cyfz@scau.edu.cn.
South China Agricultural University · CNNanyang Normal University · CN

Funding

National Natural Science Foundation of China 31372373National Natural Science Foundation of China 31372381Natural Science Foundation of Guangdong Province 2016A030311018
6 · The paper itself

Abstract

The classical baculovirus display system (BDS) has often recruited fields including gene delivery, gene therapy, and the genetic engineering of vaccines, as it is capable of presenting foreign polypeptides on the membranes of recombinant baculovirus through a transmembrane protein. However, classical BDS's high cost, complicated operation, low display efficiency and its inability to simultaneously display multiple gene products impede its practicality. In this study, we present a novel and highly efficient display system based on ires-dependent gp64 for rescuing gp64-null Bacmid of baculovirus construction without affecting the viral replication cycle, which we name the baculovirus multigene display system (BMDS). Laser scanning confocal microscopy demonstrated that eGFP, eYFP, and mCherry were translocated on the membrane of Spodoptera frugiperda 9 cell successfully as expected. Western blot analysis further confirmed the presence of the fluorescent proteins on the budded, mature viral particles. The results showed the display efficiency of target gene on cell surface is fourfold that of classical BDS. In addition, a recombinant baculovirus displaying three kinds of fluorescent proteins simultaneously was constructed, thereby demonstrating the effectiveness of BMDS as a co-display system.

Indexed as

AnimalsBaculoviridaeCell Surface Display TechniquesGene Expression Regulation, ViralInsect VirusesSpodopteraVaccinesVaccinesBaculovirus multigene display systemGene expressionHigh efficiencyInsect virus

Identifiers

PMID29923069
OpenAlexW2809058580

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.