ArticleScientific reports2018
Transcriptome analysis of microRNA156 overexpression alfalfa roots under drought stress.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Unearthing the hidden organ: vital role of the root in drought tolerance of plants.Plant molecular biology · 2025Review
- Analysis of the Genes from Gibberellin, Jasmonate, and Auxin Signaling Under Drought Stress: A Genome-Wide Approach in Castor Bean (Plants (Basel, Switzerland) · 2025Article
- Molecular Mechanisms of Alfalfa Response to Abiotic Stresses.Plants (Basel, Switzerland) · 2025Review
- Characterization of tae-miR156(s) and their response to abiotic stress in wheat (Triticum aestivum L.).BMC plant biology · 2024Article
- Ectopic expression of choline oxidase (3 Biotech · 2024Article
- Genetic and Epigenetic Responses of Autochthonous Grapevine Cultivars from the 'Epirus' Region of Greece upon Consecutive Drought Stress.Plants (Basel, Switzerland) · 2023Article
- Vegetative phase change causes age-dependent changes in phenotypic plasticity.The New phytologist · 2023Article
- MicroRNA: A Dynamic Player from Signalling to Abiotic Tolerance in Plants.International journal of molecular sciences · 2023Review
- Epigenomics in stress tolerance of plants under the climate change.Molecular biology reports · 2023Review
- Transcriptome and GWAS Analyses Reveal Candidate Gene for Root Traits of Alfalfa during Germination under Salt Stress.International journal of molecular sciences · 2023Article
- TheInternational journal of molecular sciences · 2023Article
- GWAS of adventitious root formation in roses identifies a putative phosphoinositide phosphatase (SAC9) for marker-assisted selection.PloS one · 2023Article
- Comparative Transcriptome and Anatomic Characteristics of Stems in Two Alfalfa Genotypes.Plants (Basel, Switzerland) · 2022Article
- Reference nodule transcriptomes forPlant direct · 2022Article
- Differentially expressed genes related to plant height and yield in two alfalfa cultivars based on RNA-seq.PeerJ · 2022Article
- A Genome-Wide Association Study Coupled With a Transcriptomic Analysis Reveals the Genetic Loci and Candidate Genes Governing the Flowering Time in Alfalfa (Frontiers in plant science · 2022Article
- Roles of microRNAs in abiotic stress response and characteristics regulation of plant.Frontiers in plant science · 2022Review
- Non-coding RNAs fine-tune the balance between plant growth and abiotic stress tolerance.Frontiers in plant science · 2022Review
- Non-Coding RNAs in Response to Drought Stress.International journal of molecular sciences · 2021Review
- Genome-Wide Association Analysis Coupled With Transcriptome Analysis Reveals Candidate Genes Related to Salt Stress in Alfalfa (Frontiers in plant science · 2021Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drought is one of the major abiotic stresses that negatively impact alfalfa growth and productivity. The role of microRNA156 (miR156) in drought has been demonstrated in plants. To date, there are no published studies investigating the role of miR156 in regulating global gene expression in alfalfa under drought. In our study, alfalfa genotypes overexpressing miR156 (miR156OE) exhibited reduced water loss, and enhanced root growth under drought. Our RNA-seq data showed that in response to drought, a total of 415 genes were upregulated and 169 genes were downregulated specifically in miR156OE genotypes. Genotypic comparison revealed that 285 genes were upregulated and 253 genes were downregulated in miR156OE genotypes relative to corresponding WT under drought. Gene Ontology enrichment analysis revealed that the number of differentially expressed genes belonging to biological process, molecular function and cell component functional groups was decreased in miR156OE genotypes under drought. Furthermore, RNA-Seq data showed downregulation of a gene encoding WD40 repeat in a miR156-specific manner. 5' RACE experiments verified cleavage of WD40-2 transcript under drought. Moreover, alfalfa plants overexpressing WD40-2 showed drought sensitive, whereas those with silenced WD40-2 exhibited drought tolerant phenotypes. These findings suggest that miR156 improves drought tolerance in alfalfa by targeting WD40-2.
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