ArticleThe Journal of biological chemistry2018
Loading of malonyl-CoA onto tandem acyl carrier protein domains of polyunsaturated fatty acid synthases.
Article in The Journal of biological chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Thraustochytrid PUFA synthase ER domains form a stable heterodimer.Journal of structural biology: X · 2026Article
- Charting the Biosynthetic Landscape of Hybrid Polyketide-Nonribosomal Peptide-Specialized Lipids.JACS Au · 2026Article
- Acetyl- and malonyl-CoA availability drive EPA selectivity in polyketide synthase-engineered Yarrowia lipolytica.Microbial cell factories · 2026Article
- Prokaryotic PfaB is a terminal acyltransferase that determines the final PUFA product.Protein science : a publication of the Protein Society · 2026Article
- Integrated metabolome-transcriptome analyses reveal key pathways regulating staminate catkin development and pollen maturation inFrontiers in plant science · 2025Article
- Light-Induced Flavonoid Biosynthesis inPlants (Basel, Switzerland) · 2023Article
- Integrated Metabolites and Transcriptomics at Different Growth Stages Reveal Polysaccharide and Flavonoid Biosynthesis inInternational journal of molecular sciences · 2022Article
- Article
- Transcriptional regulation mechanism of flavonoids biosynthesis gene during fruit development inFrontiers in genetics · 2022Article
- Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms.Frontiers in bioengineering and biotechnology · 2022Review
- Progress of potential drugs targeted in lipid metabolism research.Frontiers in pharmacology · 2022Review
- Genetic Suppression of Lethal Mutations in Fatty Acid Biosynthesis Mediated by a Secondary Lipid Synthase.Applied and environmental microbiology · 2021Article
- Genetic regulation of the bacterial omega-3 polyunsaturated fatty acid biosynthesis pathway.Journal of bacteriology · 2020Article
- Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the MutantMicroorganisms · 2020Article
- Artificial covalent linkage of bacterial acyl carrier proteins for fatty acid production.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Omega-3 polyunsaturated fatty acids (PUFA) are produced in some unicellular organisms, such as marine gammaproteobacteria, myxobacteria, and thraustochytrids, by large enzyme complexes called PUFA synthases. These enzymatic complexes resemble bacterial antibiotic-producing proteins known as polyketide synthases (PKS). One of the PUFA synthase subunits is a conserved large protein (PfaA in marine proteobacteria) that contains three to nine tandem acyl carrier protein (ACP) domains as well as condensation and modification domains. In this work, a study of the PfaA architecture and its ability to initiate the synthesis by selecting malonyl units has been carried out. As a result, we have observed a self-acylation ability in tandem ACPs whose biochemical mechanism differ from the previously described for type II PKS. The acyltransferase domain of PfaA showed a high selectivity for malonyl-CoA that efficiently loads onto the ACPs domains. These results, together with the structural organization predicted for PfaA, suggest that this protein plays a key role at early stages of the anaerobic pathway of PUFA synthesis.
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