ArticleBMC molecular biology2018
Laser capture microdissection for transcriptomic profiles in human skin biopsies.
Article in BMC molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- [Chinese Expert Consensus on the Clinical Practice of Non-small Cell Lung Cancer Fusion Gene Detection Based on RNA-based NGS].Zhongguo fei ai za zhi = Chinese journal of lung cancer · 2023Article
- DV200 Index for Assessing RNA Integrity in Next-Generation Sequencing.BioMed research international · 2020Article
- Laser-capture microdissection of murine lung for differential cellular RNA analysis.Cell and tissue research · 2019Article
- Standardization of organoid culture for evaluation of melanogenesis induced by UVB, UVA and visible light.Anais brasileiros de dermatologiaArticle
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
backgroundThe acquisition of reliable tissue-specific RNA sequencing data from human skin biopsy represents a major advance in research. However, the complexity of the process of isolation of specific layers from fresh-frozen human specimen by laser capture microdissection, the abundant presence of skin nucleases and RNA instability remain relevant methodological challenges. We developed and optimized a protocol to extract RNA from layers of human skin biopsies and to provide satisfactory quality and amount of mRNA sequencing data.
resultsThe protocol includes steps of collection, embedding, freezing, histological coloration and relative optimization to preserve RNA extracted from specific components of fresh-frozen human skin biopsy of 14 subjects. Optimization of the protocol includes a preservation step in RNALater
conclusionsThe described implemented and optimized protocol can be used for generating transcriptomics data on skin tissues, and it is potentially applicable to other tissues. It can be extended to multicenter studies, due to the introduction of an initial step of preservation of the specimen that allowed the shipment of biological samples.
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