ArticlePloS one2018
Whole blood transcriptome changes following controlled human malaria infection in malaria pre-exposed volunteers correlate with parasite prepatent period.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 3 of them syntheses that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 3 syntheses or guidelines pooled it, 41 citations in OpenAlex.
- Host Transcriptional Meta-signatures Reveal Diagnostic Biomarkers for Plasmodium falciparum Malaria.The Journal of infectious diseases · 2024Pooled it
- RNA-seq research landscape in Africa: systematic review reveals disparities and opportunities.European journal of medical research · 2023Pooled it
- Multicohort transcriptome analysis of whole blood identifies robust human response signatures in Plasmodium falciparum infections.Malaria journal · 2022Pooled it
- Trial
- Transcriptomic analyses of differentially expressed human genes, micro RNAs and long-non-coding RNAs in severe, symptomatic and asymptomatic malaria infection.Scientific reports · 2024Article
- Entire expressed peripheral blood transcriptome in pediatric severe malarial anemia.Nature communications · 2024Article
- The malarial blood transcriptome: translational applications.Biochemical Society transactions · 2024Review
- Systems analysis and controlled malaria infection in Europeans and Africans elucidate naturally acquired immunity.Nature immunology · 2021Article
- Dual RNA Sequencing Meta-analysis inmSystems · 2021Article
- Host and Parasite Transcriptomic Changes upon Successive Plasmodium falciparum Infections in Early Childhood.mSystems · 2020Article
- Decoding the complexities of human malaria through systems immunology.Immunological reviews · 2020Review
- Use of gene expression studies to investigate the human immunological response to malaria infection.Malaria journal · 2019Review
- Transcriptome profiling reveals functional variation in Plasmodium falciparum parasites from controlled human malaria infection studies.EBioMedicine · 2019Article
- Whole-blood transcriptomic signatures induced during immunization by chloroquine prophylaxis and Plasmodium falciparum sporozoites.Scientific reports · 2019Article
- Transmission-Blocking Vaccines: Old Friends and New Prospects.Infection and immunity · 2019Review
- Blood transcriptome profile induced by an efficacious vaccine formulated with salivary antigens from cattle ticks.NPJ vaccines · 2019Article
Corrections and comments
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Authors and funding
13 authors at 5 institutions in 3 countries.
Funding
Abstract
Malaria continues to be one of mankind's most devastating diseases despite the many and varied efforts to combat it. Indispensable for malaria elimination and eventual eradication is the development of effective vaccines. Controlled human malaria infection (CHMI) is an invaluable tool for vaccine efficacy assessment and investigation of early immunological and molecular responses against Plasmodium falciparum infection. Here, we investigated gene expression changes following CHMI using RNA-Seq. Peripheral blood samples were collected in Bagamoyo, Tanzania, from ten adults who were injected intradermally (ID) with 2.5x104 aseptic, purified, cryopreserved P. falciparum sporozoites (Sanaria® PfSPZ Challenge). A total of 2,758 genes were identified as differentially expressed following CHMI. Transcriptional changes were most pronounced on day 5 after inoculation, during the clinically silent liver phase. A secondary analysis, grouping the volunteers according to their prepatent period duration, identified 265 genes whose expression levels were linked to time of blood stage parasitemia detection. Gene modules associated with these 265 genes were linked to regulation of transcription, cell cycle, phosphatidylinositol signaling and erythrocyte development. Our study showed that in malaria pre-exposed volunteers, parasite prepatent period in each individual is linked to magnitude and timing of early gene expression changes after ID CHMI.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.