ArticleThe Journal of allergy and clinical immunology2019
A novel role for neutrophils in IgE-mediated allergy: Evidence for antigen presentation in late-phase reactions.
Article in The Journal of allergy and clinical immunology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- Role of Neutrophils on the Ocular Surface.International journal of molecular sciences · 2021Pooled it
- Neutrophils in cancer.Nature reviews. Cancer · 2026Review
- Breaking the bottleneck of asthma treatment: the future of omni-targeted therapy.Journal of advanced research · 2026Review
- Analysis and validation of diagnostic biomarkers and immune cell infiltration characteristics in chronic spontaneous urticaria and autophagy based on machine learning.The World Allergy Organization journal · 2026Article
- Beyond the Neutrophil Count: Functional Profiling and Targeted Modulation of Neutrophils in Paediatric Care.Journal of innate immunity · 2026Review
- Complexes of allergen and specific IgG antibodies activate human neutrophils.Frontiers in allergy · 2026Article
- <italic>Streptococcus pyogenes</italic> Infection and CD4Journal of innate immunity · 2026Article
- A Mixture of Four Probiotic Strains (Probiatop®) Mitigates Food Allergy to Ovalbumin in Mice.Probiotics and antimicrobial proteins · 2025Article
- Keratinocyte-neutrophil interactions revealed as targetable drivers of sustained inflammation in Sweet syndrome.The Journal of clinical investigation · 2025Article
- Absence of neutrophils impairs the host defense in murine footpad model of chromoblastomycosis.PLoS neglected tropical diseases · 2025Article
- The role of neutrophils in allergic disease.Clinical and experimental immunology · 2025Review
- [Research progress of immune cell markers in allergic rhinitis for the evaluation of allergen immunotherapy].Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery · 2024Review
- How Neutrophils Shape the Immune Response: Reassessing Their Multifaceted Role in Health and Disease.International journal of molecular sciences · 2023Review
- Upregulated expression of Notch1/4 - JAG-1/DLL-1 detected in allergic rhinitis.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2023Article
- Neuroimmune interactions in atopic and allergic contact dermatitis.The Journal of allergy and clinical immunology · 2023Review
- Article
- Human neutrophils require short exposure to cytokines and allergen to become functional antigen-presenting cells.Allergy · 2023Article
- Neutrophils in autoimmunity: when the hero becomes the villain.Clinical and experimental immunology · 2022Article
- Review
- Neuronal-Immune Cell Units in Allergic Inflammation in the Nose.International journal of molecular sciences · 2022Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundNeutrophils and allergen-specific T cells accumulate in patients with allergic late-phase reactions (LPRs). Their presence is associated with severe inflammation. Cytokines, such as GM-CSF, IFN-γ, and IL-3, which are typically found in patients with allergic LPRs, have been proposed to convert neutrophils into antigen-presenting cells (APCs).
objectiveWe sought to assess the antigen-processing and antigen-presenting capacities of neutrophils from allergic patients.
methodsNeutrophils were isolated from peripheral blood of donors with birch pollen allergy and stimulated with GM-CSF, IFN-γ, and IL-3. The viability and expression of HLA-DR, CD80, and CD86 were assessed by using flow cytometry. HLA-DM expression was analyzed by means of immunoblotting. Allergen uptake was studied after fluorescence labeling of the major birch pollen allergen Bet v 1. Bet v 1 was digested with neutrophilic endolysosomal extracts, and the resulting fragments were sequenced by using mass spectrometry. Neutrophils were used as APCs in coculture experiments with autologous HLA-DR-restricted and Bet v 1-specific T-cell clones reactive with epitopes in different regions of the allergen. In all experiments monocytes were used for comparison. Fluids from suction blisters formed on top of LPRs induced by using intradermal allergen injection were assessed for HLA-DR
resultsThe cytokines significantly enhanced the survival, allergen uptake, and expression of HLA-DM and HLA-DR on neutrophils. Neutrophils rapidly degraded Bet v 1 into fragments containing all relevant T-cell epitopes. Cytokine-activated, allergen-pulsed neutrophils induced proliferative and cytokine responses of Bet v 1-specific T cells irrespective of epitope specificity, confirming that they fully processed and presented the allergen. HLA-DR
conclusionNeutrophils can serve as APCs for local allergen-specific effector T cells in patients with allergic LPRs.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.