Evidence map›Paper›PMID 29916184›Full record

ArticleMetabolic brain disease2018

Antibiotics protect against EAE by increasing regulatory and anti-inflammatory cells.

Hilary A Seifert, Gil Benedek, Ha Nguyen, Grant Gerstner, Ying Zhang, Gail Kent, Arthur A Vandenbark, Jürgen Bernhagen, Halina Offner

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Metabolic brain disease, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
1.5field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

28 citing papers in PubMed, 37 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 3 countries.

Hilary A SeifertDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA.
Gil BenedekDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA.
Ha NguyenDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA.
Grant GerstnerDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA.
Ying ZhangDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA.
Gail KentDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA.
Arthur A VandenbarkDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA.
Jürgen BernhagenVascular Biology, Institute for Stroke and Dementia Research, Klinikum der Universität München, Ludwig-Maximilians-University Munich, Munich, Germany.
Halina OffnerDepartment of Neurology, Oregon Health & Science University, Portland, OR, USA. offnerva@ohsu.edu.ORCID 0000-0001-6472-6079
Oregon Health & Science University · USMunich Cluster for Systems Neurology · DE

Funding

Estrogen-Induced Regulatory B Cells Protect Against EAE & Limit CNS InflammationR01NS080890 · NINDS · OREGON HEALTH & SCIENCE UNIVERSITY · PI OFFNER, HALINA · 2013 to 2017
$1.6M
NINDS NIH HHS R01 NS080890NINDS NIH HHS R01NS080890
6 · The paper itself

Abstract

A seven day pretreatment course of an oral antibiotic cocktail (Ampicillin, Metronidazole, Neomycin Sulfate, and Vancomycin) was shown to induce changes in peripheral immune regulation and protect mice from signs of experimental autoimmune encephalomyelitis (EAE). To determine if a shorter course of antibiotic pretreatment could also protect the mice from EAE and induce regulatory immune cells, studies were conducted using the same oral antibiotic cocktail for three days. In addition, the CNS was examined to determine the effects of antibiotic pretreatment on EAE disease course and immune modulation within the affected tissue. The shorter three day pretreatment course was also significantly protective against severe EAE in C57BL/6 mice. Moreover, our study found increased frequencies of regulatory cells and a decrease in the frequency of anti-inflammatory macrophages in the spleen of EAE protected mice. Additionally, a chemokine and chemokine receptor array run on mRNA from spinal cords revealed that genes associated with regulatory T cells and macrophage recruitment were strongly upregulated in the antibiotic pretreated mice. Additional RT-PCR data showed genes associated with anti-inflammatory microglia/macrophages were upregulated and pro-inflammatory genes were downregulated. This suggests the macrophages recruited to the spinal cord by chemokines are subsequently polarized toward an anti-inflammatory phenotype. These results lend strong support to the conclusion that a three day course of antibiotic treatment given prior to the induction of severe EAE profoundly protected the mice by inducing regulatory lymphocytes in the periphery and an anti-inflammatory milieu in the affected spinal cord tissue.

Indexed as

ImmunomodulationAnimalsAnti-Bacterial AgentsB-LymphocytesChemokinesDown-RegulationEncephalomyelitis, Autoimmune, ExperimentalFemaleLymph NodesMacrophagesMiceMice, Inbred C57BLMicrogliaReceptors, ChemokineSpinal CordSpleenAnti-Bacterial AgentsChemokinesReceptors, ChemokineAntibioticCNSEAEMicrobiotaNeuroinflammationRegulatory cells

Identifiers

PMID29916184
PMCPMC6298859
OpenAlexW2808914056

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.