ArticleMethods in molecular biology (Clifton, N.J.)2018
Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians.
Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed.
- Multiplexed gene expression analysis in dissociated and intact planarian flatworm tissues using in situ hybridization chain reaction.Biology open · 2026Article
- Developmental Toxicity of Ibrutinib: Insights from Stem Cell Dynamics and Neural Regeneration in Planarians.Biomolecules · 2025Article
- Article
- Article
- Coral-algal endosymbiosis characterized using RNAi and single-cell RNA-seq.Nature microbiology · 2023Article
- Defined diets for freshwater planarians.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- Formalizing Phenotypes of Regeneration.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Set1 Targets Genes with Essential Identity and Tumor-Suppressing Functions in Planarian Stem Cells.Genes · 2021Article
- Direct Current Electric Stimulation Alters the Frequency and the Distribution of Mitotic Cells in Planarians.Bioelectricity · 2021Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Efforts to elucidate mechanisms of regeneration in the planarian Schmidtea mediterranea have included the application of immunocytochemical methods to detect specific molecules and label cells and tissues in situ. Here we describe methods for immunofluorescent labeling of whole mount planarians. We outline protocols for fixation and steps for processing animals prior to immunolabeling, incorporating commonly utilized reagents for mucus removal, pigment bleaching, tissue permeabilization, and antigen retrieval. Because processing steps can mask or degrade antigens, we also recommend protocol parameters that can be tested simultaneously to optimize sample preparation for novel antibodies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.