ArticlePeerJ2018
Can non-destructive DNA extraction of bulk invertebrate samples be used for metabarcoding?
Article in PeerJ, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Three sampling strategies reveal spatial and methodological patterns in macroinvertebrate diversity in freshwater habitats.iScience · 2026Article
- Bycatch in a bottle: what taxa are recoverable from metabarcoding DNA in historical invertebrate collection preservative fluid?The Journal of heredity · 2026Article
- Accuracy of occurrence and abundance estimates from insect metabarcoding.bioRxiv : the preprint server for biology · 2026Article
- Fast-tracking bespoke DNA reference database generation from museum collections for biomonitoring and conservation.Molecular ecology resources · 2025Article
- A non-destructive, fast, inexpensive, non-toxic chelating resin-based DNA extraction protocol for insect voucher specimens and associated microbiomes.Journal of insect science (Online) · 2025Article
- Maximizing Identification Precision of Hymenoptera and Brachycera (Diptera) With a Non-Destructive DNA Metabarcoding Approach.Ecology and evolution · 2025Article
- Environmental gradients and optimal fixation time revealed with DNA metabarcoding of benthic sample fixative.Scientific reports · 2024Article
- Optimizing insect metabarcoding using replicated mock communities.Methods in ecology and evolution · 2023Article
- Development of an array of molecular tools for the identification of khapra beetle (Trogoderma granarium), a destructive beetle of stored food products.Scientific reports · 2023Article
- Haplotype-level metabarcoding of freshwater macroinvertebrate species: A prospective tool for population genetic analysis.PloS one · 2023Article
- Article
- FAVIS: Fast and versatile protocol for non-destructive metabarcoding of bulk insect samples.PloS one · 2023Article
- Repeated subsamples during DNA extraction reveal increased diversity estimates in DNA metabarcoding of Malaise traps.Ecology and evolution · 2022Article
- Article
- Resurrection and redescription ofZooKeys · 2022Article
- Article
- Modelling technical and biological biases in macroinvertebrate community assessment from bulk preservative using multiple metabarcoding markers.Molecular ecology · 2021Article
- Developing a non-destructive metabarcoding protocol for detection of pest insects in bulk trap catches.Scientific reports · 2021Article
- Pooling size sorted Malaise trap fractions to maximize taxon recovery with metabarcoding.PeerJ · 2021Article
- Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh throughput DNA sequencing of bulk invertebrate samples or metabarcoding is becoming increasingly used to provide profiles of biological communities for environmental monitoring. As metabarcoding becomes more widely applied, new reference DNA barcodes linked to individual specimens identified by taxonomists are needed. This can be achieved through using DNA extraction methods that are not only suitable for metabarcoding but also for building reference DNA barcode libraries.
methodsIn this study, we test the suitability of a rapid non-destructive DNA extraction method for metabarcoding of freshwater invertebrate samples.
resultsThis method resulted in detection of taxa from many taxonomic groups, comparable to results obtained with two other tissue-based extraction methods. Most taxa could also be successfully used for subsequent individual-based DNA barcoding and taxonomic identification. The method was successfully applied to field-collected invertebrate samples stored for taxonomic studies in 70% ethanol at room temperature, a commonly used storage method for freshwater samples. DISCUSSION: With further refinement and testing, non-destructive extraction has the potential to rapidly characterise species biodiversity in invertebrate samples, while preserving specimens for taxonomic investigation.
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