ArticleFrontiers in plant science2018
An Efficient Protocol for Model Legume Root Protoplast Isolation and Transformation.
Article in Frontiers in plant science, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Establishment of efficient hypocotyl-derived protoplast isolation and its application in soybean (Frontiers in plant science · 2025Article
- A cargo sorting receptor mediates chloroplast protein trafficking through the secretory pathway.The Plant cell · 2024Article
- The S-acylation cycle of transcription factor MtNAC80 influences cold stress responses in Medicago truncatula.The Plant cell · 2024Article
- Optimization of preparation and transformation of protoplasts from Populus simonii × P. nigra leaves and subcellular localization of the major latex protein 328 (MLP328).Plant methods · 2024Article
- Isolation, Purification, and Application of Protoplasts and Transient Expression Systems in Plants.International journal of molecular sciences · 2023Review
- RPG interacts with E3-ligase CERBERUS to mediate rhizobial infection in Lotus japonicus.PLoS genetics · 2023Article
- Formin-mediated bridging of cell wall, plasma membrane, and cytoskeleton in symbiotic infections of Medicago truncatula.Current biology : CB · 2021Article
- An Optimized Transformation System and Functional Test ofInternational journal of molecular sciences · 2021Article
- Highly Efficient Leaf Base Protoplast Isolation and Transient Expression Systems for Orchids and Other Important Monocot Crops.Frontiers in plant science · 2021Article
- Development of a Rapid Selection System for Salt-Resistant Mutants ofPlants (Basel, Switzerland) · 2020Article
- Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions.Plants (Basel, Switzerland) · 2020Review
- From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming.International journal of molecular sciences · 2020Review
- Development of a rapid and efficient protoplast isolation and transfection method for chickpea (MethodsX · 2020Article
- Loading calcium fluorescent probes into protoplasts to detect calcium in the flesh tissue cells ofHorticulture research · 2020Article
- Atypical Receptor Kinase RINRK1 Required for Rhizobial Infection But Not Nodule Development inPlant physiology · 2019Article
- Agroinfiltration-based efficient transient protein expression in leguminous plants.Plant biotechnology (Tokyo, Japan) · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transient gene expression systems using protoplasts have been widely used for rapid functional characterization of genes and high-throughput analysis in many model and crop species. Here, we describe a simplified and highly efficient root protoplast isolation and transient expression system in the model legumes
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