ArticleScientific reports2018
Aberration correction considering curved sample surface shape for non-contact two-photon excitation microscopy with spatial light modulator.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Model-based aberration corrected microscopy inside a glass tube.Journal of microscopy · 2025Article
- Spatial light modulation for interferometric scattering microscopy.Journal of microscopy · 2025Article
- Large-scale cranial window for in vivo mouse brain imaging utilizing fluoropolymer nanosheet and light-curable resin.Communications biology · 2024Article
- Adaptive aberration correction using an electrowetting array.Applied physics letters · 2023Article
- Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.Nature communications · 2022Article
- Protocol for constructing an extensive cranial window utilizing a PEO-CYTOP nanosheet forSTAR protocols · 2021Article
- Learned adaptive multiphoton illumination microscopy for large-scale immune response imaging.Nature communications · 2021Article
- Adaptive Optical Two-Photon Microscopy for Surface-Profiled Living Biological Specimens.ACS omega · 2021Article
- PEO-CYTOP Fluoropolymer Nanosheets as a Novel Open-Skull Window for Imaging of the Living Mouse Brain.iScience · 2020Article
- Pathological application of carbocyanine dye-based multicolour imaging of vasculature and associated structures.Scientific reports · 2020Article
- Adaptive optics microspectrometer for cross-correlation measurement of microfluidic flows.Journal of biomedical optics · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, excitation light wavefront modulation is performed considering the curved sample surface shape to demonstrate high-quality deep observation using two-photon excitation microscopy (TPM) with a dry objective lens. A large spherical aberration typically occurs when the refractive index (RI) interface between air and the sample is a plane perpendicular to the optical axis. Moreover, the curved sample surface shape and the RI mismatch cause various aberrations, including spherical ones. Consequently, the fluorescence intensity and resolution of the obtained image are degraded in the deep regions. To improve them, we designed a pre-distortion wavefront for correcting the aberration caused by the curved sample surface shape by using a novel, simple optical path length difference calculation method. The excitation light wavefront is modulated to the pre-distortion wavefront by a spatial light modulator incorporated in the TPM system before passing through the interface, where the RI mismatch occurs. Thus, the excitation light is condensed without aberrations. Blood vessels were thereby observed up to an optical depth of 2,000 μm in a cleared mouse brain by using a dry objective lens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.