ArticleProceedings of the National Academy of Sciences of the United States of America2018
Three-dimensional virtual histology of human cerebellum by X-ray phase-contrast tomography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 156 citations in OpenAlex.
- Paired CycleGAN-based virtual staining for 3D X-ray histology of bone-implant systems.Journal of synchrotron radiation · 2026Article
- Label-free 3D virtual histology of human formalin-fixed paraffin-embedded (FFPE) prostate needle biopsies with propagation-based phase-contrast micro-CT (PBCT).bioRxiv : the preprint server for biology · 2026Article
- Critical point drying of brain tissue for X-ray phase-contrast imaging.Journal of synchrotron radiation · 2026Article
- Quantitative Stain Mapping in X-Ray Virtual Histology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Three-dimensional high-content imaging of unstained soft tissue with subcellular resolution using a laboratory-based X-ray microscope.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- X-ray waveguide optics at GINIX/P10 PETRA III: recent progress and future directions.Journal of synchrotron radiation · 2026Article
- Demonstration of a family of X-ray dark-field retrieval approaches on a common set of samples.Journal of synchrotron radiation · 2026Article
- Nondestructive X-ray tomography of brain tissue ultrastructure.Nature methods · 2025Article
- 3D histology of human heart-forming organoids by X-ray phase-contrast tomography.Communications biology · 2025Article
- Mass spectrometric imaging of organic and metallic metabolites by plasmon-induced interfacial charge-transfer transition (PICTT) on Au sputtered ITO slides.Chemical science · 2025Article
- Synchrotron Radiation-Based Tomography of an Entire Mouse Brain with Sub-Micron Voxels: Augmenting Interactive Brain Atlases with Terabyte Data.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Air artifact suppression in phase contrast micro-CT using conditional generative adversarial networks.Journal of synchrotron radiation · 2025Article
- Scaling up X-ray holographic nanotomography for neuronal tissue imaging.Biomedical optics express · 2025Article
- 3D multiscale characterization of the human placenta: Bridging anatomy and histology by X-ray phase-contrast tomography.PNAS nexus · 2025Article
- Synchrotron X-ray imaging of soft biological tissues - principles, applications and future prospects.Journal of cell science · 2024Review
- Ortho-positronium lifetime for soft-tissue classification.Scientific reports · 2024Article
- Volumetric changes of the enteric nervous system under physiological and pathological conditions measured using x-ray phase-contrast tomography.JGH open : an open access journal of gastroenterology and hepatology · 2024Article
- 3D imaging of SARS-CoV-2 infected hamster lungs by X-ray phase contrast tomography enables drug testing.Scientific reports · 2024Article
- Phase contrast micro-CT with adjustable in-slice spatial resolution at constant magnification.Physics in medicine and biology · 2024Article
- A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging.Journal of synchrotron radiation · 2024Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To quantitatively evaluate brain tissue and its corresponding function, knowledge of the 3D cellular distribution is essential. The gold standard to obtain this information is histology, a destructive and labor-intensive technique where the specimen is sliced and examined under a light microscope, providing 3D information at nonisotropic resolution. To overcome the limitations of conventional histology, we use phase-contrast X-ray tomography with optimized optics, reconstruction, and image analysis, both at a dedicated synchrotron radiation endstation, which we have equipped with X-ray waveguide optics for coherence and wavefront filtering, and at a compact laboratory source. As a proof-of-concept demonstration we probe the 3D cytoarchitecture in millimeter-sized punches of unstained human cerebellum embedded in paraffin and show that isotropic subcellular resolution can be reached at both setups throughout the specimen. To enable a quantitative analysis of the reconstructed data, we demonstrate automatic cell segmentation and localization of over 1 million neurons within the cerebellar cortex. This allows for the analysis of the spatial organization and correlation of cells in all dimensions by borrowing concepts from condensed-matter physics, indicating a strong short-range order and local clustering of the cells in the granular layer. By quantification of 3D neuronal "packing," we can hence shed light on how the human cerebellum accommodates 80% of the total neurons in the brain in only 10% of its volume. In addition, we show that the distribution of neighboring neurons in the granular layer is anisotropic with respect to the Purkinje cell dendrites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.